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Steering trajectories of rolling cells by 2D asymmetric receptor patterning

机译:二维不对称受体图案在滚动细胞的转向中的作用

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We demonstrate a simple method to make high-resolution P-selectin patterns based on microcontact printing for controlling cell rolling. SEM characterization revealed well-defined patterns that could direct the trajectories of rolling HL60 cells along the edges. Cell tracking revealed that velocities of rolling cells were higher at the edges as compared to plain P-selectin regions, with a maximum at an edge angle of 20°. The distance traveled by the HL60 cells along the edges decreased with increasing edge angles. These substrates may be used to tune cell adhesion and control the transport of the cells for separation and analysis.
机译:我们演示了一种基于微接触印刷来控制细胞滚动的高分辨率P-选择蛋白图案的简单方法。 SEM表征揭示了定义明确的模式,该模式可以指导滚动的HL60细胞沿边缘运动。单元格跟踪显示,与普通的P-选择素区域相比,边缘上的滚动单元的速度更高,在20°的边角处最大。 HL60细胞沿边缘行进的距离随边缘角度的增加而减小。这些底物可用于调节细胞粘附并控制细胞的运输以进行分离和分析。

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