Expression, cross-linking and characterization of recombinant chitin binding resilin

机译：重组几丁质结合弹性蛋白的表达，交联和表征

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Resilin is a polymeric rubber-like protein secreted by insects to specialized cuticle regions, in areas where high resilience and low stiffness are required. Resilin binds to the cuticle polysaccharide chitin via a chitin binding domain and is further polymerized through oxidation of the tyrosine residues resulting in the formation of dityrosine bridges and assembly of a high-performance protein-carbohydrate composite material. We describe for the first time a comprehensive study on the mechanical, structural and biochemical function of chitin binding recombinant Drosophila melanogaster resilin. Various resilin constructs were cloned including the full length gene enabling Ni-NTA purification, as well as heat and salt precipitation for rapid and efficient purification. The binding isotherms and constants (K

机译：弹性蛋白（Resilin）是一种聚合的橡胶状蛋白质，被昆虫分泌到需要高回弹力和低刚度的区域中的特殊表皮区域。弹性蛋白通过甲壳质结合域与表皮多糖甲壳质结合，并通过酪氨酸残基的氧化而进一步聚合，导致二酪氨酸桥的形成和高性能蛋白质-碳水化合物复合材料的组装。我们第一次描述了几丁质结合重组果蝇果蝇resilin的机械，结构和生化功能的全面研究。克隆了多种弹性蛋白构建体，包括能够进行Ni-NTA纯化的全长基因，以及用于快速有效纯化的热和盐沉淀法。结合等温线和常数（K

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《IEEE Annual Northeast Bioengineering Conference》|2010年|P.1-2|共2页
会议地点 New York NY(US)
作者
Guokui Qin; Lapidot S.; Numata K.; Xiao Hu; Meirovitch S.; Dekel M.; Podoler I.; Shoseyov O.; Kaplan D.L.;
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Dept. of Biomed. Eng. Tufts Univ. Medford MA USA;

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1. Expression, Cross-Linking, and Characterization of Recombinant Chitin Binding Resilin [J] . Guokui Qin, Shaul Lapidot, Keiji Numata Biomacromolecules . 2009,第12期

机译：重组几丁质结合弹性蛋白的表达，交联和表征
2. Expression and characterization of an enhanced recombinant heparinase I with chitin binding domain [J] . Xu Shuqin, Qiu Meiling, Zhang Xuanyue, International Journal of Biological Macromolecules: Structure, Function and Interactions . 2017,第Pta1期

机译：用邻丁蛋白结合结构域的增强重组肝素酶I的表达与表征
3. Expression and purification of recombinant cytoplasmic domain of human erythrocyte band 3 with hexahistidine tag or chitin-binding tag in Escherichia coli [J] . Ding Y., Jiang WH., Su Y., Protein Expression and Purification . 2004,第2期

机译：具有六组氨酸标签或几丁质结合标签的人红细胞带3重组胞质域的表达和纯化
4. Expression, cross-linking and characterization of recombinant chitin binding resilin [C] . Guokui Qin, Shaul Lapidot, Keiji Numata, Annual Northeast Bioengineering Conference . 2010

机译：重组甲壳素结合resilin的表达，交联和表征
5. Cloning, Expression, Purification and Characterization of Heparin-Binding Pocket of Recombinant FGF1 [D] . ?Ashraf, Quratulayn 2020

机译：克隆，表达，纯化及性质肝素结合重组 FGF1 的口袋
6. Expression and Characterization of the Chitin-Binding Domain of Chitinase A1 from Bacillus circulans WL-12 [O] . Masayuki Hashimoto, Takahisa Ikegami, Shizuka Seino, 2000

机译：圆形芽孢杆菌WL-12的几丁质酶A1的几丁质结合结构域的表达和表征
7. Production and characterization of recombinant tachycitin, the Cys-rich chitin-binding protein [O] . Tetsuya Suetake, Tomoyasu Aizawa, Nozomi Koganesawa, 2002

机译：重组特性霉素的生产和表征，富含Cys-富含Chitin结合蛋白
8. Expression, Production and Characterization of Recombinant HumanAcetylcholinesterase and Its Mutants [R] . Shafferman, A. 1993

机译：重组人乙酰胆碱酯酶及其突变体的表达，产生和鉴定

Expression, cross-linking and characterization of recombinant chitin binding resilin

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