• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>IEEE Annual Northeast Bioengineering Conference >Patterning micro-stiffness in cell-adhesive substrate using microfluidics-based lithography
【24h】

Patterning micro-stiffness in cell-adhesive substrate using microfluidics-based lithography

机译：使用基于微流控的光刻技术在细胞粘附基质中图案化微刚度

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study, we demonstrate the formation of 3D cell-adhesive hydrogels exhibiting well-defined spatial variation in stiffness using our previously developed microfluidics-based lithography technique. PEG monoacrylate-linked bovine fibrinogen (PEG-fibrinogen) is photopolymerized into specific, user-defined shapes inside a microchannel, and successive cycles of fabrication result in a heterogeneous structure with controlled regional variations in stiffness. Atomic force microscope (AFM) indentations were used to directly confirm the micro-stiffness distribution, and morphological and migratory behavior of cells in microenvironments of controlled variations in stiffness was characterized. Our approach allows control of microscale variations of stiffness in cell-adhesive substrates with high precision and flexibility and offers the opportunity to examine differential cell-ECM interactions relevant to a multitude of fundamental cellular processes.
机译：在这项研究中，我们证明了使用我们以前开发的基于微流控技术的光刻技术，可以形成显示出明确定义的空间刚度变化的3D细胞粘附水凝胶。 PEG单丙烯酸酯连接的牛纤维蛋白原（PEG-纤维蛋白原）在微通道内被光聚合成用户定义的特定形状，并且连续的制造周期导致异质结构的刚度受到区域控制。原子力显微镜（AFM）压痕用于直接确认微刚度分布，并表征了在刚性受控的微环境中细胞的形态和迁移行为。我们的方法可以高精度和灵活地控制细胞粘附基质中刚度的微观变化，并提供了检查与众多基本细胞过程相关的细胞-ECM相互作用的机会。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号