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Towards optical cell transfection inside a micro flow cell

机译：进行微流通池内的光学细胞转染

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摘要

For optical transfection, cells are shortly subjected to intense, focused laser radiation which leads to a temporary opening in the cell membrane. Although the method is very efficient and ensures high cell viability, the targeting of single cells with laser pulses is a tedious and slow approach. We present first measurements aiming at an experimental setup which is suitable for high throughput and automated optical cell transfection. In our setup, cells flow through a micro flow cell where they are spatially confined. The laser radiation is focused into the cell in a way that an elongated focal region is realized. This makes the time consuming aiming of the laser beam at individual cells unnecessary and opens the possibility to develop a completely automated system. The elongated laser focal region is realized by a quasi-Bessel beam which is generated by an axicon lens setup and continuously scanned from side to side of the cell. We present test measurements of the newly employed setup and discuss its suitability to be fully integrated into a flow cell sequencing system.
机译：为了进行光学转染，细胞会短期受到强烈的聚焦激光辐射，这会导致细胞膜上的临时开口。尽管该方法非常有效并确保了高细胞活力，但是用激光脉冲靶向单个细胞是一种乏味且缓慢的方法。我们目前针对的是针对高通量和自动化光学细胞转染的实验装置的首次测量。在我们的设置中，细胞流经微流动池，并在空间上受到限制。激光辐射以实现细长聚焦区域的方式聚焦到细胞中。这使得激光束不必费时地对准各个单元，并且开辟了开发全自动系统的可能性。细长的激光聚焦区域由准贝塞尔光束实现，准贝塞尔光束由轴锥透镜设置生成，并从像元的一侧到另一侧连续扫描。我们介绍了新近使用的装置的测试测量结果，并讨论了将其完全集成到流通池测序系统中的适用性。

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  • 来源
  • 会议地点 San Francisco CA(US)
  • 作者

    H.G. Breunig; A. Uchugonova; K. Koenig;

  • 作者单位

    Saarland University, Faculty of Mechatronics and Physics, Department of Biophotonics and Laser Technology, Campus A5.1, 66123 Saarbruecken, Germany,JenLab GmbH, Schillerstr 1, 07745 Jena, Germany and Science Park 2, Campus D1.2, 66123 Saarbruecken, Germany;

    Saarland University, Faculty of Mechatronics and Physics, Department of Biophotonics and Laser Technology, Campus A5.1, 66123 Saarbruecken, Germany,JenLab GmbH, Schillerstr 1, 07745 Jena, Germany and Science Park 2, Campus D1.2, 66123 Saarbruecken, Germany;

    Saarland University, Faculty of Mechatronics and Physics, Department of Biophotonics and Laser Technology, Campus A5.1, 66123 Saarbruecken, Germany,JenLab GmbH, Schillerstr 1, 07745 Jena, Germany and Science Park 2, Campus D1.2, 66123 Saarbruecken, Germany;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    optical transfection; transfection; optoporation; flow cell; Bessel beam;

    机译：光学转染；转染对抗流通池贝塞尔光束;

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