Optical tweezers force calibration using a fast-shuttering camera

机译：光学镊子使用快速快门相机强制校准

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We report using a fast-shuttering CCD camera to determine the transverse stiffness of an optical tweezers trap. By utilizing the relation between the particle position variance and the trap stiffness we are able to circumvent the sampling requirements of the position power spectrum approach. We find that for trap stiffness of order 10~(-5) N/m exposures shorter than 0.001 sec seem to be required in order to give valid measurements. For longer exposures (～0.01 sec) the trap stiffness can be overestimated by a factor of three or more.

机译：我们报告使用快速快门CCD相机来确定光镊陷阱的横向刚度。通过利用粒子位置方差与陷阱刚度之间的关系，我们可以规避位置功率谱方法的采样要求。我们发现，对于陷阱强度为10〜（-5）N / m的陷阱，似乎需要短于0.001秒的时间才能进行有效的测量。对于更长的曝光时间（〜0.01秒），阱的刚度可能被高估了三倍或更多。

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《Imaging, Manipulation, and Analysis of Biomolecules, Cells and Tissues V; Progress in Biomedical Optics and Imaging; vol.8 no.18; Proceedings of SPIE-The International Society for Optical Engineering; vol.6441》|2007年|644114.1-644114.8|共8页
会议地点 San JoseCA(US)
作者
J. P. Sharpe; Claudia Iniguez-Palomares; Rafael Jimenez-Flores;
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作者单位

Dept Physics, Cal Poly State University, San Luis Obispo, CA 93407;

Centro de Investigacion en Alimentacion y Desarrollo, Hermosillo, Sonora, Mexico;

Dairy Products Technology Center, Cal Poly State University, SLO, CA 93407;

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原文格式 PDF
正文语种 eng
中图分类光、激光生物医学;
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专利

1. Absolute calibration of forces in optical tweezers [J] . R. S. Dutra, N. B. Viana, P. A. Maia Neto, Physical Review, A. Atomic, molecular, and optical physics . 2014,第1aPta2期

机译：镊子中力的绝对校准
2. CALIBRATION OF LIGHT FORCES IN OPTICAL TWEEZERS [J] . Felgner H., Schliwa M., Muller O. Applied optics . 1995,第6期

机译：光学镊子中的光力校准
3. Calibration of force detection for arbitrarily shaped particles in optical tweezers [J] . Ann A. M. Bui, Anatolii V. Kashchuk, Marie Anne Balanant, Scientific reports. . 2018,第1期

机译：校准光镊中任意形状的颗粒的力
4. Optical tweezers force calibration using a fast-shuttering camera [C] . J. P. Sharpe, Claudia Iniguez-Palomares, Rafael Jimenez-Flores Conference on Imaging, Manipulation, and Analysis of Biomolecules, Cells and Tissues . 2007

机译：光学镊子使用快速快门的相机强制校准
5. Focus, edge detection, and CCD camera characterization for development of an optical overlay calibration standard. [D] . Fox, Stephen Harris. 2000

机译：聚焦，边缘检测和CCD相机特性描述，用于开发光学叠加校准标准。
6. New Detector Sensitivity Calibration and the Calculation of the Interaction Force between Particles Using an Optical Tweezer [O] . Pavel Yale, Jean-Michel Edoukoua Konin, Michel Abaka Kouacou, 2018

机译：新的探测器灵敏度校准和使用光镊计算粒子之间的相互作用力
7. Stretching Single DNA Molecules and using High-Speed Camera Power Spectral Analysis to Demonstrate High Force Capabilities of Holographic Optical Tweezers [O] . van der Horst Astrid, Farré Arnau, Forde Nancy R. 2010

机译：拉伸单个DNA分子并使用高速相机功率谱分析证明全息光镊的高抗力能力

Optical tweezers force calibration using a fast-shuttering camera

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