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Effects of Amniotic Membrane Extraction on Rabbit Corneas with Herpes Simplex Keratitis

机译：羊膜提取物对单纯疱疹性角膜炎兔角膜的影响。

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摘要

The amniotic membrane exhibits many biological properties that may be helpful in treating ocular surface diseases. In this study, we investigated the effect of topical amniotic membrane extraction on herpes simplex keratitis (HSK) in a rabbit model. The corneas of rabbits were infected with herpes simplex type 1 virus. The rabbits were divided into 2 groups according to topical treatment methods: saline and AM extraction. The corneal lesions and angiogenesis severity were measured and graded at 0,1,3,5,7, and 14 days after treatment. Histologic examination was performed and the expression of interleukin (IL)-2, IL-10 and vascular endothelial growth factor (VEGF) were determined by enzyme-linked immunosorbent assay at 7 or 14 days after treatment. The severity of keratitis treated with AM extraction was less than saline treated eyes after 5 days of treatment (P<0.05). The extent of angiogenesis in the eyes of AM extraction treatment revealed greater reduction than the control ones after 3 days of treatment (P<0.05). The amount of IL-2 and VEGF were decreased after treatment of AM extraction, whereas the amount of IL-10 was increased. The present study showed the extraction from fresh human amniotic membrane dramatically decreased the corneal inflammation and angiogenesis in experimental HSK by modulating the expression of proinflammatory cytokines and VEGF in the infected corneas.
机译：羊膜具有许多生物学特性，可能有助于治疗眼表疾病。在这项研究中，我们调查了局部羊膜提取对兔子模型中单纯疱疹性角膜炎（HSK）的影响。兔子的角膜感染了1型单纯疱疹病毒。根据局部治疗方法将兔子分为两组：盐水和AM提取。在治疗后0、1、3、5、7和14天测量角膜病变和血管新生的严重程度并分级。进行组织学检查，并在治疗后7或14天通过酶联免疫吸附法测定白介素（IL）-2，IL-10和血管内皮生长因子（VEGF）的表达。治疗5天后，经AM提取治疗的角膜炎的严重程度低于生理盐水治疗的眼睛（P <0.05）。治疗3天后，AM提取物治疗组眼中的血管生成程度显示出比对照组更大的减少（P <0.05）。 AM提取液处理后，IL-2和VEGF的含量减少，而IL-10的含量增加。本研究表明，通过调节感染的角膜中促炎性细胞因子和VEGF的表达，从新鲜的人羊膜提取物可显着降低实验性HSK中的角膜炎症和血管生成。

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