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首页> 外文会议>International meeting on chemical sensors >Electron transfer between redox enzymes and electrodes through the artificial redox proteins and its applciatin for biosensors
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Electron transfer between redox enzymes and electrodes through the artificial redox proteins and its applciatin for biosensors

机译：氧化还原酶和电极之间通过人工氧化还原蛋白进行电子转移及其在生物传感器中的应用

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摘要

Three kinds of artifical redox proteins have been designed and prepared in order to discuss the applicability of them for the facile electron transferrign interfaces between redox enzxymes and electrode materials.At first,ferrocene derivative-modified bovine serum albumin was prepared and was adsorbed together with fructose dehydrogenase on the conductive SnO_2 electrode to fabricate an enzyme sensor for furtose.Secondly,avidins which were chemically modified with anthraquinonyl group or pyrroloquinoline quinone were prepared.They were assembled onto biotin-modified gold electrode surfaces and teir redox properties at the surface-bound state were electrochemcially studied.At third,the genetically enineered streptavidin into which a redox-active nonatural amino acid,anthraquinonylalanine was incorporated at a specific site was synthesized and characterized as an artifical redox protein.
机译：设计并制备了三种人工氧化还原蛋白，以探讨它们在氧化还原酶和电极材料之间的便捷电子传递界面上的适用性。首先，制备了二茂铁衍生物修饰的牛血清白蛋白，并与果糖一起吸附其次，制备了用蒽醌基或吡咯并喹啉醌化学修饰的亲和素，并将其组装在生物素修饰的金电极表面上，并在表面结合状态下具有氧化还原特性。第三，合成了遗传修饰的链霉亲和素，其中在特定位点掺入了氧化还原活性的天然氨基酸蒽醌基丙氨酸，并将其表征为人工氧化还原蛋白。

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