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首页> 外文会议>International Plant Nutrition Colloquium >Functional study of the phyA gene using an Agrobacterium tumefaciens-mediated Medicago truncatula transformation system
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Functional study of the phyA gene using an Agrobacterium tumefaciens-mediated Medicago truncatula transformation system

机译：用根癌农杆菌介导的run藜转化系统对phyA基因进行功能研究

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In this study, Agrobacterium strain EHA105 containing pCAMBIA1301 was used to treat a Medicago truncatula genotype, A17. The percentage of callus induction was very low because of Agrobacterium overgrowth. There was only about 21% induction for embryo calli and embryos also were few. This result confirmed that Medicago truncatula A17 is difficult to transform and regenerate via somatic embryogenesis. It is necessary to use high frequent regenerative Medicago genotypes to establish an efficient transformation system in order to study the expression and function of the phyA gene.
机译：在这项研究中，含有pCAMBIA1301的农杆菌EHA105菌株被用于治疗苜蓿苜蓿（Medicago truncatula）基因型A17。由于农杆菌过度生长，愈伤组织诱导的百分比非常低。胚愈伤组织仅诱导约21％，并且胚也很少。该结果证实了截叶苜蓿A17很难通过体细胞胚发生转化和再生。为了研究phyA基因的表达和功能，有必要使用高频率的再生苜蓿基因型来建立有效的转化系统。

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