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首页> 外文会议>International Academic Conference on Environmeatal amp; Occupational Medicine; 20041110-12; Shanghai(CN) >Anti-lipid Peroxidation and Protection of Liver Mitochondria Against Injuries by Picroside Ⅱ
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Anti-lipid Peroxidation and Protection of Liver Mitochondria Against Injuries by Picroside Ⅱ

机译：抗性脂质过氧化作用和苦参碱Ⅱ对肝线粒体的保护作用

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[Objective]To investigate of anti — lipid peroxidation and protection effects of picroside Ⅱ on liver mitochondria against injuries in mice with liver damage. [Methods] Three animal models of liver damage induced by carbon tetrachloride (CCl_4 :0.1 ml/10 g, ip), D-galactosamine (D-GalN: 500 mg/kg, ip) and acetaminophen(AP: 0.15g/kg, ip) respectively were treated with various concentrations of Picroside Ⅱ(5, 10, 20 mg/kg, ig), then we chose continuously monitoring method (recommended by International Clinical Chemistry League) to analyze serum alanine aminotransferase(ALT) and aspartate aminotransferase(AST) values, Marland method to detect the activitiy of manganese-superoxide dismutase (SOD) in liver mitochondria, TBA colorimetry to determine the content of malonic aldehyde (MDA) in liver tissue, DTNB method to evaluate the activity of glutathioneperoxidase (GSH-Px) and Lowry method to detect protein level in liver tissue. Meanwhile, effects of picroside Ⅱ on the activity of ATPase and swell extent of mitochondria in hepatocytes damaged by AP were also evaluated. [Results] Picroside Ⅱ could significantly prevent liver toxicity in the three models of liver damage. It decreased the high levels of ALT and AST in serum induced by the administration of CCl_4, D-GalN and AP, reduced the cellular damage of liver markedly, and appeared to be even more potent than the positive control drug of biphenyl dimethyl dicarboxylate pilules (DDB) . In groups treated with different doses of Picroside Ⅱ, compared to the model group, the content of MDA in serum decreased evidently, whereas the content of SOD and GSH-Px increased in a dose-dependent manner, and the difference beared evident statistical significance (P < 0.01). Further, in the study of AP model, picroside Ⅱ inhibited AP-induced liver toxicity in mice, enhanced the activity of ATPase, improved the swell extent of mitochondria and helped maintain a normal balance of energy metabolism. [Conclusion]Picroside Ⅱ can evidently relieve the hepatocyte injuries induced by CCl_4, D-GalN and AP, help scavenge free radicals, protect normal constructions of mitochondria membrane and enhance the activitiy of ATPase in mitochondria, thereby modulating the balance of liver energy metabolism, which might be part of the mechanisms of Picroside Ⅱ's hepatoprotective effects.
机译：［目的］研究苦瓜苷Ⅱ的抗脂质过氧化作用及其对肝线粒体损伤的保护作用。 ［方法］采用三氯化碳（CCl_4：0.1 ml / 10 g，ip），D-半乳糖胺（D-GalN：500 mg / kg，ip）和对乙酰氨基酚（AP：0.15g / kg）诱导的三种肝损伤动物模型。 ip）分别用不同浓度的PicrosideⅡ（5、10、20 mg / kg，ig）处理，然后选择连续监测方法（国际临床化学联盟推荐）分析血清丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（ip） AST）值，Marland方法检测肝线粒体中的超氧化物歧化酶（SOD）活性，TBA比色法确定肝组织中丙二醛（MDA）的含量，DTNB方法评估谷胱甘肽过氧化物酶（GSH-Px）的活性）和Lowry方法检测肝组织中的蛋白质水平。同时，评价了苦瓜苷Ⅱ对AP损伤肝细胞ATP酶活性和线粒体肿胀程度的影响。 [结果]在三种肝损伤模型中，苦瓜苷Ⅱ均具有明显的预防肝毒性作用。它降低了CCl_4，D-GalN和AP的给药引起的血清ALT和AST的高水平，显着减轻了肝脏的细胞损伤，甚至比联苯二甲基二羧酸二甲酯微丸的阳性对照药物更有效（ DDB）。与模型组相比，不同剂量的吡ro糖苷Ⅱ治疗组的血清MDA含量明显降低，而SOD和GSH-Px的含量呈剂量依赖性增加，且差异具有统计学意义（ P <0.01）。此外，在AP模型的研究中，苦瓜苷Ⅱ抑制了AP诱导的小鼠肝毒性，增强了ATPase的活性，改善了线粒体的溶胀程度，并帮助维持了能量代谢的正常平衡。 ［结论］ PicrosideⅡ能明显缓解CCl_4，D-GalN和AP诱导的肝细胞损伤，帮助清除自由基，保护线粒体膜的正常结构，增强线粒体中ATPase的活性，从而调节肝脏能量代谢的平衡，这可能是PicrosideⅡ的保肝作用机制的一部分。

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