A Rapid, Simple and Efficient Method for PCR-mediated Amplification and Labeling of Gene of Interest in Bacterial Cell

机译：PCR介导的细菌细胞扩增和标记的快速，简单，高效方法

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Picked a single bacterial colony transformed with gene of interest from a solid agar plate, and used specific primers to directly amplify and label genes of interest by Polymerase Chain Reaction. This method has many advantages, without tremendous labor of bacterium liquid culture, extraction of plasmid and digestion with restriction enzyme.

机译：从固体琼脂平板上挑选一个用目的基因转化的细菌菌落，并使用特异性引物通过聚合酶链反应直接扩增和标记目的基因。该方法具有许多优点，无需费力地进行细菌液体培养，提取质粒和用限制酶消化。

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来源
《International Agro-Biotechnology Conference; 2005; Heilongjiang(CN)》|2005年|P.341-345|共5页
会议地点 Heilongjiang(CN)
作者
WANG Ji-hua; LI Zhi-yu; YANG Ru-bing; JIANG Lu-lu; WANG Hai-yang;
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作者单位

School of Life and Environment Science, Harbin Normal University Harbin 150080,China;

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会议组织
原文格式 PDF
正文语种 eng
中图分类农业生物学;
关键词
polymerase; Chain; reaction; primed in situ labeling; PCR-DIG labeling; PCR-Southern;

机译：聚合酶链反应原位标记PCR-DIG标记PCR-Southern;

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专利

1. Rapid and sensitive detection of Citrus Bacterial Canker by loop-mediated isothermal amplification combined with simple visual evaluation methods [J] . Luciano A Rigano, María R Marano, Atilio P Castagnaro, BMC Microbiology . 2010,第1期

机译：环路介导的等温扩增结合简单的视觉评估方法快速灵敏地检测柑橘细菌性溃疡病
2. Rapid and Simple Methods for Labeling White Blood Cells and Platelets with Indium-111-Oxine [J] . Frank G. Steffel, Shyam A. Rao Journal of nuclear medicine technology . 1987,第2期

机译：用铟-111-氧标记白细胞和血小板的快速简便方法
3. Simple and Rapid Visual Detection Methods of Orf Virus by B2L Gene based Loop-Mediated Isothermal Amplification Assay [J] . Gnanavel Venkatesan, Veerakyathappa Bhanuprakash, Vinayagamurthy Balamurugan, Advances in Animal and Veterinary Sciences . 2016,第3期

机译：基于B2L基因的环介导等温扩增法简便快速地检测Orf病毒
4. A Rapid, Simple and Efficient Method for PCR-mediated Amplification and Labeling of Gene of Interest in Bacterial Cell [C] . WANG Ji-hua, LI Zhi-yu, YANG Ru-bing, International Agro-Biotechnology Conference; 2005; Heilongjiang(CN) . 2005

机译：PCR介导的细菌细胞扩增和标记的快速，简单，高效方法
5. An efficient algorithm combining cell multipole and multigrid methods for rapid evaluation of dipole iteration in polarizable force fields. [D] . Dinh-Truong, Thuy Linh. 2006

机译：一种将单元多极和多网格方法相结合的有效算法，可快速评估可极化力场中的偶极子迭代。
6. Rapid and sensitive detection of Citrus Bacterial Canker by loop-mediated isothermal amplification combined with simple visual evaluation methods [O] . Luciano A Rigano, María R Marano, Atilio P Castagnaro, 2010

机译：环介导的等温扩增结合简单的视觉评估方法快速灵敏地检测柑橘细菌性溃疡病
7. Rapid and sensitive detection of Citrus Bacterial Canker by loop-mediated isothermal amplification combined with simple visual evaluation methods [O] . Luciano A Rigano, María R Marano, Atilio P Castagnaro, 2010

机译：环介导的等温扩增结合简单的视觉评估方法快速灵敏地检测柑橘细菌性溃疡病
8. A Comparative Evaluation of Techniques for Rapid and Efficient in vivo Labeling of Red Cells with (99mTc) Pertechnetate. [R] . Hamilton, R. G., Alderson, P. O. 1977

机译：（99mTc）高锝酸盐快速有效体内标记红细胞技术的比较评价。

A Rapid, Simple and Efficient Method for PCR-mediated Amplification and Labeling of Gene of Interest in Bacterial Cell

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