Semi-automated Mapping of Cell Nuclei in 3D-Stacks from Optical-Sectioning Microscopy

机译：半自动映射的3D堆栈中的细胞核从光学切片显微镜。

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3D-stacks of optical sections through the vertebrate retina with fluorescent stained cell nuclei were measured with a laser scanning microscope. The evaluation of the data volumes with dedicated digital imaging algorithms gives access to complex morphometric tissue-characters that are discussed in terms of functional morphology. The thickness of nuclear layers and the 3D-coordinates of cell nuclei are detected automatically to measure cell densities, cell ratios and to create character-distribution-maps of the entire retina.

机译：用激光扫描显微镜测量穿过脊椎动物视网膜的光学切片的3D堆栈，并显示荧光染色的细胞核。使用专用的数字成像算法对数据量进行评估可以访问复杂的形态学组织特征，这些特征在功能形态方面进行了讨论。自动检测核层的厚度和细胞核的3D坐标，以测量细胞密度，细胞比例并创建整个视网膜的字符分布图。

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来源
《International Conference on Advances in Mass Data Analysis of Signals and Images in Medicine Biotechnology and Chemistry(MDA 2006/2007); 200707018; Leipzig(DE)》|2007年|P.156164|共2页
会议地点 Leipzig(DE)
作者
Martin Hess;
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作者单位

Biozentrum der LMU Muenchen, Grosshadernerstr. 2, 82152 Planegg, Germany;

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会议组织
原文格式 PDF
正文语种 eng
中图分类信息处理（信息加工）;生物医学工程;
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4. Semi-automated Mapping of Cell Nuclei in 3D-Stacks from Optical-Sectioning Microscopy [C] . Martin Hess International Conference on Advances in Mass Data Analysis of Signals and Images in Medicine Biotechnology and Chemistry(MDA 2006/2007) . 2007

机译：从光学切片显微镜从3D堆叠中的细胞核自动化映射
5. Mechanical characterization of aortic valve interstitial cells and their nuclei using atomic force microscopy. [D] . Liu, Haijiao. 2012

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机译：过滤富集的循环肿瘤细胞的半自动显微镜检查方法

Semi-automated Mapping of Cell Nuclei in 3D-Stacks from Optical-Sectioning Microscopy

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