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首页> 外文会议>International Conference on Environmental Toxicology; 20060911-13; Mykonos(GR) >Bioindication of microcystins toxicity by germinating seeds
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Bioindication of microcystins toxicity by germinating seeds

机译：通过发芽种子生物指示微囊藻毒素的毒性

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摘要

The effects of microcystins, produced by Microcystis aeruginosa PCC 7820, on seed germination, seedling growth, activity of selected enzymes and membrane permeability were evaluated, in order to elaborate the biotests for natural solution toxicity indication in water reservoirs. Commercial seeds of four selected species were allowed to germinate at 20℃ on filter paper moistened with: (ⅰ) water (control), (ⅱ) three concentrations of microcystins cultures produced by Microcystis aeruginosa PCC 7820 or (ⅲ) medium BG11 in which these cultures were conducted. To evaluate the sensitivity of these seeds to microcystins the number of the germinated seeds were counted and the length of roots and hypocotyls were measured 1, 2 and 3 days after sowing, while activity of RN-ase, phosphatase (pH 6.0 and pH 7.5), dehydrogenases and electrolyte leakage were measured after 8 and 24 hours of seed exposition in these compounds. The results showed that microcystins did not decrease significantly germination percentage and mean germination time of the tested seeds, except of L. sativa where the number of the germinated seeds was slightly reduced. A toxic effect of these solutions was exhibited by decreased root length in all germinated seeds, while hypocotyls length was not significantly affected by them. Microcystins also decreased the activity of the investigated enzymes and increased electrolyte leakage measured 8 and 24 hours after sowing. The research showed that growth of roots and activity of RN-ase, phosphatase (pH 6.0 and pH 7.5) or dehydrogenases, as well as electrolyte leakage from germinating seeds of selected species could be used for fast monitoring of the microcystin toxicity in water.
机译：评估了铜绿微囊藻PCC 7820产生的微囊藻毒素对种子发芽，幼苗生长，所选酶的活性和膜通透性的影响，以详细说明水库中天然溶液毒性指示的生物测试。使四个选定物种的商业种子在滤纸上在20℃下萌发，滤纸上沾满：（ⅰ）水（对照），（ⅱ）三种浓度的铜绿微囊藻PCC 7820产生的微囊藻培养物或（ⅲ）BG11培养基，文化进行。为了评估这些种子对微囊藻毒素的敏感性，计算了发芽种子的数量，并在播种后1、2和3天测量了根和下胚轴的长度，同时测定了RN酶，磷酸酶（pH 6.0和pH 7.5）的活性。在这些化合物中，种子暴露8和24小时后，测定了脱氢酶和电解质的渗漏。结果表明，除了紫花苜蓿发芽的种子数量略有减少外，微囊藻毒素并没有显着降低被测种子的发芽率和平均发芽时间。这些溶液的毒性作用表现为所有发芽种子的根长减小，而胚轴长度不受它们的显着影响。微囊藻毒素还降低了所研究酶的活性，并增加了播种后8和24小时测得的电解质渗漏。研究表明，根的生长和RNase，磷酸酶（pH 6.0和pH 7.5）或脱氢酶的活性以及所选物种发芽种子的电解质渗漏可用于快速监测水中的微囊藻毒素毒性。

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