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首页> 外文会议>International Conference on Food Factors(ICoFF 03); 20031201-04; Tokyo(JP) >Isolation and identification of lactic acid bacteria with effect of immune protection to Eschericia coli in mice
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Isolation and identification of lactic acid bacteria with effect of immune protection to Eschericia coli in mice

机译：乳酸菌的分离和鉴定对小鼠具有免疫保护作用

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Lactic acid bacteria were isolated from an alcohol fermentation broth, and the activity as a probiotic was examined using pathogenic E. coli. Thirty-six strains exhibiting good growth were isolated in the medium of concentrated mush which was a residue resulted in the alcohol distillation process. One of these strains, Lactobacillus paracasei subsp. paracasei I-5, could be grown in the medium containing 8 vol% ethanol and at 45℃. The characteristics were different from the type strain, L. paracasei subsp. paracasei NBRC 15889~T. L. paracasei I-5 showed an excellent growth in the concentrated mush, which just diluted two-fold and adjusted the pH. ICR mice were fed with a standard germ-free feed (CMF) and the strain I-5 (7 x 10~9 cells/day) was orally administrated for 11 days prior to the intraperitoneal challenge with pathogenic E. coli Juhl. After the challenge, mice administrated the strain I-5 exhibited a high survival rate and survival extension days (p < 0.01) compared with the control. The results suggested that the strain might enhance the animal resistance against microbial pathogens. Neonatal diarrhea caused by E. coli is a serious disease in calf breeding. The strain might be practically valuable to prevent diarrhea in calves.
机译：从酒精发酵液中分离乳酸菌，并使用致病性大肠杆菌检查其作为益生菌的活性。在浓糊状培养基中分离出表现出良好生长的36个菌株，其为乙醇蒸馏过程中的残留物。这些菌株之一，副干酪乳杆菌亚种。副干酪I-5，可以在含有8％（体积）乙醇且温度为45℃的培养基中生长。特征与典型菌株副干酪乳杆菌亚种不同。副干酪NBRC 15889〜T。副干酪乳杆菌I-5在浓糊糊中表现出出色的生长，仅稀释两倍就可以调节pH。给ICR小鼠喂食标准的无菌饲料（CMF），并在用病原性大肠杆菌Juhl腹膜内攻击之前口服I-5株（7 x 10〜9个细胞/天），持续11天。攻击后，与对照相比，施用I-5品系的小鼠表现出较高的存活率和存活延长天数（p <0.01）。结果表明，该菌株可能增强动物对微生物病原体的抵抗力。大肠杆菌引起的新生儿腹泻是小牛育种中的严重疾病。该菌株在预防小牛腹泻方面可能具有实用价值。

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