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首页> 外文会议>International Conference on Livestock Services Proceedings >IDENTIFICATION OF BACTERIAL INOCULANT BY 16S AND 26S REBOSOMAL DNA VIA POLYMERASE CHAIN REACTION
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IDENTIFICATION OF BACTERIAL INOCULANT BY 16S AND 26S REBOSOMAL DNA VIA POLYMERASE CHAIN REACTION

机译：聚合酶链反应通过16S和26S重组体DNA鉴定细菌接种物

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摘要

The conservation of herbage biomass with the aid of fermentative bacteria to produce silage for animal feedstuff is widely used. During ensilage, the anaerobic microbial conversion of fermentable carbohydrates to organic acids leads to a reduction of the pH value and thus to a preservation of plant compounds. An array of lactic acid bacteria (LAB), such as Lactobacillus plantarum, Streptococcus lactis, Enterococcus feacalis, Pediococcus pentosaceus, one kind of Bacillus subtilis for fiber decomposed, one kind of Candida utilis for organic acid, are used to buildup the ensiling bacterial inoculant. Ensiling plant material with the aid of ensiling bacterial inoculant is a common agricultural practice for conserving forages independently of the time point of harvest. In order to identify the species-specific, 16S ribosomal DNA (rDNA) sequence for the LAB and Bacillus subtilis, 26S ribosomal DNA (rDNA) sequence for the Candida utilis are obtained via polymerase chain reaction (PCR). Through the analysis of the sequences, a phyligenetic tree of the 16S rDNA region of 5 kinds bacteria was plotted.
机译：借助于发酵细菌来生产用于动物饲料的青贮饲料来保护草本生物质已被广泛使用。在青贮过程中，可发酵碳水化合物向有机酸的厌氧微生物转化导致pH值降低，从而保护了植物化合物。一系列乳酸菌（LAB），例如植物乳杆菌，乳酸链球菌，粪肠球菌，戊糖戊球菌，一种用于纤维分解的枯草芽孢杆菌，一种用于有机酸的枯草芽孢杆菌，用于积累细菌接种物。 。借助于使细菌接种物包裹的方法使植物材料堆积是一种不依赖收获时间点而保护草料的常见农业实践。为了鉴定LAB和枯草芽孢杆菌的物种特异性16S核糖体DNA（rDNA）序列，通过聚合酶链反应（PCR）获得了utilis假丝酵母的26S核糖体DNA（rDNA）序列。通过序列分析，绘制了5种细菌的16S rDNA区域的系统树。

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