• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Conference on Monitoring of Toxic Chemical and Biomarkers >Immunologic methods for monitoring carcinogen exposure
【24h】

Immunologic methods for monitoring carcinogen exposure

机译：监测致癌物暴露的免疫学方法

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract: Immunologic methods have been developed for monitoring human exposure to environmental and occupational carcinogens. These methods involve the development of monoclonal and polyclonal antisera which specifically recognize the carcinogens themselves or their DNA or protein adducts. Antisera recognizing the DNA adducts of several polycyclic aromatic hydrocarbon diol epoxides have been used in competitive enzyme-linked immunosorbent assays to monitor adducts in tissue or blood samples. Elevated levels of DNA adducts have been seen in mononuclear cells of smokers and in total white blood cells of foundry and coke oven workers. Environmental exposure to PAH has been measured in individuals living in a highly polluted region of Poland. Antisera recognizing PAH-DNA adducts have also been used in immunohistochemical studies to monitor adducts in specific cells of biopsy samples. The DNA adducts of aflatoxin B$-1$/ have been monitored in liver tissue of hepatocellular carcinoma patients in Taiwan. Detectable adducts were seen in 50 - 70% of the patients suggesting that dietary exposure to this carcinogen may be a risk factor for cancer induction. Thus, immunoassays for monitoring exposure to carcinogens are an important tool in epidemiologic studies.!45
机译：摘要：已经开发出免疫学方法来监测人类对环境和职业致癌物的暴露。这些方法涉及单克隆和多克隆抗血清的开发，它们可以特异性识别致癌物本身或它们的DNA或蛋白质加合物。识别几种多环芳烃二醇环氧化合物的DNA加合物的抗血清已用于竞争性酶联免疫吸附测定中，以监测组织或血液样品中的加合物。在吸烟者的单核细胞以及铸造厂和炼焦炉工人的总白细胞中，DNA加合物的水平升高。已对生活在波兰高污染地区的个人进行了PAH环境暴露测量。识别PAH-DNA加合物的抗血清也已用于免疫组化研究中，以监测活检样品特定细胞中的加合物。在台湾肝细胞癌患者的肝组织中，黄曲霉毒素B $ -1 $ /的DNA加合物已被监测。在50-70％的患者中发现了可检测到的加合物，表明饮食中接触这种致癌物可能是诱发癌症的危险因素。因此，用于监测致癌物暴露的免疫测定是流行病学研究的重要工具！45

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号