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首页> 外文会议>International Conference on Monitoring of Toxic Chemical and Biomarkers >Monoclonal antibody-based enzyme immunoassays for the sensitive detection of s-triazines in water
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Monoclonal antibody-based enzyme immunoassays for the sensitive detection of s-triazines in water

机译：基于单克隆抗体的酶免疫分析法可灵敏地检测水中的S-三嗪

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Abstract: Immunoassays in pesticide residue analysis significantly profit from the monoclonal antibody (mAb) technology because a sufficient supply of standardized antibodies can be provided. For the production of atrazine-specific mAbs hybridoma cells were produced by fusion of mouse myeloma cells and spleen cells from mice which were immunized with 4-chloro-6-ethylamino- 1,3,5-triazine-2-(6-aminohexanoic acid) coupled to keyhole limped hemocyanin. After screening with a competitive enzyme immunoassay (EIA) a mAb with high binding affinity towards atrazine was selected. A sensitive EIA was developed detecting atrazine with a range from 0.05 to 1 $mu@g/l with a test midpoint of 0.1 $mu@g/l. The mAb cross-reacts predominantly with propazine (136%). Since this herbicide is not used in most European countries, the test allows a rapid and inexpensive screening for atrazine in the ppt range. Another EIA has been constructed for the detection of terbuthylazine. The limiting factor in EIA development is the screening for cell lines secreting mAbs with high affinity and selectivity towards the analyte. Super paramagnetic beads being coated with suitable immonoconjugates are shown to bind to hybridomas presenting hapten-specific receptors on their surface. Hybridomas secreting hapten-specific mAbs can be removed by a magnet and be cloned subsequently by standard procedures. A considerable demand of mAbs is expected in the future due to new emerging techniques such as immunosensors.!9
机译：摘要：农药残留分析中的免疫分析可从单克隆抗体（mAb）技术中获得巨大收益，因为可以提供足够的标准化抗体。为了产生阿特拉津特异性mAb，通过用4-氯-6-乙基氨基-1,3,5-三嗪-2-（6-氨基己酸）免疫的小鼠骨髓瘤细胞和小鼠脾细胞融合来产生杂交瘤细胞。 ）再加上锁孔血红蛋白。用竞争性酶免疫法（EIA）筛选后，选择对阿特拉津具有高结合亲和力的mAb。开发了一种灵敏的EIA，可检测at去津的范围为0.05至1μμg/ l，测试中点为0.1μμg/ l。 mAb主要与丙嗪（136％）发生交叉反应。由于大多数欧洲国家未使用该除草剂，因此该测试可对ppt范围内的at去津进行快速且廉价的筛查。已经构建了另一种EIA用于检测丁丁嗪。 EIA开发的限制因素是筛选分泌对分析物具有高亲和力和选择性的单克隆抗体的细胞系。涂有合适的immonoconjugates的超顺磁珠显示绑定到其表面上呈现半抗原特异性受体的杂交瘤。分泌半抗原特异性mAb的杂交瘤可以用磁铁去除，然后通过标准程序克隆。由于免疫传感器等新兴技术的出现，未来对mAb的需求预计会很大！9

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