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首页> 外文会议>International Conference on Surface and Colloid Science; 200309; Brazil >Phospholipid/triglyceride mixtures: analysis and comparison of the solubilisation profiles
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Phospholipid/triglyceride mixtures: analysis and comparison of the solubilisation profiles

机译：磷脂/甘油三酸酯混合物：溶解曲线的分析和比较

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In excess water, mixtures of phospholipids (PL) and triglyce-rides (TG) form different types of aggregates, principally vesicles and emulsion droplets, the respective amounts of which depend on R, the PL-toTG concentration ratio. When R is high, liposomes are the major aggregate species and when this ratio is low, emulsion droplets coexist with liposomes. The objective of the present study was to determine the proportion of vesicles in mixtures of low PL/-to-TG ratio. It has been previously described that time-resolved selective solubilisation of high PL-to-TG ratio mixtures with Triton X-100 allowed the detection of the presence of emulsion droplets in a liposomal suspension. The method has been adapted here by using another detergent, octylgluco-side, allowing us to obtain sharper solubilisation profiles thanks to the fact that it is a pure compound. A large range of PL-to-TG ratio mixtures were then solubilised by oc-tylglucoside and two ratios were more closely compared: 10:4, at which liposomes are the major components, and 10:40, at which emulsion droplets are the major species. For a PL-to-TG ratio of 10:4, liposomes could be selectively solubilised (which was already known), but we demonstrated that emulsion droplets could be selectively solubilised as well. Hence, we evidenced two solubilisation steps: first the liposomes and then the emulsion droplets. For a PL-to-TG ratio of 10:40, the solubilisation was more complex and depended on the initial PL concentration. An event disrupting the solubilisation profile could be identified corresponding to a temporary aggregation/fusion of the droplets owing to the destabili-sation of the water interface. Defined conditions where this event will not interfere with the solubilisation profile have to be found to precisely determine the solubilisation steps. In this way, it will be possible to achieve the quantification of the proportion of bilayers in an emulsion (low PL-to-TG ratio).
机译：在过量的水中，磷脂（PL）和甘油三酸酯（TG）的混合物形成不同类型的聚集体，主要是囊泡和乳液液滴，其各自的量取决于R，即PL与TG的浓度比。当R高时，脂质体是主要的聚集体种类，而当该比率低时，乳剂液滴与脂质体共存。本研究的目的是确定低PL / TG比例的混合物中囊泡的比例。先前已经描述了使用Triton X-100对高PL / TG比混合物进行时间分辨的选择性增溶，可以检测脂质体悬浮液中乳液液滴的存在。在这里，该方法已通过使用另一种去污剂辛基葡糖苷进行了修改，由于它是纯化合物，因此使我们可以获得更清晰的增溶特性。然后，大范围的PL与TG的比例混合物可被辛基葡糖苷溶解，并且将两个比例进行了更紧密的比较：10：4（脂质体为主要成分）和10:40（乳液液滴为主要成分）种类。对于PL与TG的比例为10：4，脂质体可以被选择性地溶解（这是已知的），但是我们证明了乳液液滴也可以被选择性地溶解。因此，我们证明了两个增溶步骤：首先是脂质体，然后是乳剂液滴。当PL与TG的比例为10:40时，溶解更复杂，并且取决于初始PL浓度。由于水界面的不稳定，对应于液滴的暂时聚集/融合，可以识别出破坏增溶曲线的事件。必须找到该事件不会干扰增溶曲线的规定条件，以精确确定增溶步骤。以此方式，可以实现乳液中双层比例的定量（低PL / TG比）。

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