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首页> 外文会议>International Conference on Surface and Colloid Science; 200309; Brazil >Atomic force microscopy in a liquid and in situ ellipsometry as complementary techniques for the study of protein adsorption
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Atomic force microscopy in a liquid and in situ ellipsometry as complementary techniques for the study of protein adsorption

机译：液体和原位椭圆光度法中的原子力显微镜作为蛋白质吸附研究的补充技术

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摘要

The combination of two in situ techniques for the study of immobilization of biomolecules is seldom performed. Therefore, the combination of in situ ellipsometry with atomic force microscopy (AFM) in a liquid was used to follow the adsorption process of enolase, an enzyme of the glycolytic pathway. The adsorbed amount and topographic information of the adsorbed layers were obtained in the liquid environment. AFM images showed that surfaces with comparable adsorbed amounts of protein presented immobilized entities arranged in strings or in spherical clumps, evidencing the importance of combining in situ ellipsometry and AFM in a liquid.
机译：很少将两种原位技术结合在一起进行生物分子的固定化研究。因此，原位椭偏法与原子力显微镜（AFM）在液体中的结合用于跟踪烯醇酶（一种糖酵解途径的酶）的吸附过程。在液体环境中获得吸附量和吸附层的形貌信息。原子力显微镜图像显示，具有可比的蛋白质吸附量的表面呈现出固定的实体，这些实体固定成串或球形团块，这证明了在液体中结合原位椭圆偏振法和原子力显微镜的重要性。

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