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首页> 外文会议>International Congress on Genes, Gene Families and Isozymes(ICGGFI); 20050917-21; Shanghai(CN) >Localization of Ovary Aromatase Gene on the Nile Tilapia Chromosome
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Localization of Ovary Aromatase Gene on the Nile Tilapia Chromosome

机译：卵巢芳香酶基因在尼罗罗非鱼染色体上的定位

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摘要

The recombinant plasmid containing ovary aromatase gene from BAC library of Nile tilapia, Oreochromis niloticus, was extracted and purified according to the instruction of QIAGEN Plasmid Mini Handbook with a little of modification. The probe of aromatase gene for the in situ hybridization was prepared from the plasmid DNA and labelled with biotin by degenerate oligo-nucleotide primed PCR (DOP-PCR). The labelled probe was then applied in the fluorescence in situ hybridization (FISH) to the mitotic metaphase chromosomes of Nile tilapia in order to localize the aromatase gene in the Nile tilapia. The result showed that the ovary aromatase gene of Nile tilapia was located on one pair of small chromosome other than the sex chromosome. So the aromatase gene is not primary sex determining gene in Nile tilapia, but its function to sex indicates that some autosomal loci can affect sex determination and differentiation in fish.
机译：按照QIAGEN Plasmid Mini Handbook的说明，经少量修改，从尼罗罗非鱼的尼罗罗非鱼的BAC文库中提取了含有卵巢芳香化酶基因的重组质粒，并进行了纯化。从质粒DNA制备用于原位杂交的芳香酶基因探针，并通过简并寡核苷酸引发的PCR（DOP-PCR）用生物素标记。然后将标记的探针通过荧光原位杂交（FISH）应用于尼罗罗非鱼的有丝分裂中期染色体，以便将芳香酶基因定位在尼罗罗非鱼中。结果表明，尼罗罗非鱼的卵巢芳香化酶基因位于性染色体以外的一对小染色体上。因此，芳香酶基因不是尼罗罗非鱼的主要性别决定基因，但其对性别的作用表明某些常染色体位点可影响鱼类的性别决定和分化。

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