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首页> 外文会议>International Forum on Progress on Post-Genome Technologies(5'IFPT); 20070910-11; Suzhou(CN) >Detection multiple SNPs in a large number of samples by Multiplex-PCR method combining gel-based DNA microarray
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Detection multiple SNPs in a large number of samples by Multiplex-PCR method combining gel-based DNA microarray

机译：结合基于凝胶的DNA芯片的Multiplex-PCR方法检测大量样品中的多个SNP

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摘要

We have developed gel-based DNA microarry to genotype single nucleotide polymorphism (SNP).Though the 3-D polyacrylamide gel-based microarray platform was a simple, robust and high-throughput methodology to detect one SNP for a large number of samples on one chip, sometimes, more SNPs need to genotype for large samples. In this research, we developed multiplex polymerase chain reaction (multiplex-PCR), coupled with the gel-based microarray which could genotype several SNP loci for large numbers of sample simultaneously. Five SNPs (rs191296, rs2280073, rs17599165, rs17599416 and rs7660336) of GABRA4 gene were detected at one time by the method. Our experiment demonstrated that multiplex-PCR combined with 3-D polyacrylamide gel-based microarray could be applied as a simple, robust, high-throughput, especially time-saving and cost-saving way for many different SNPs analysis in a large number of individuals.
机译：我们已经开发出了基于凝胶的DNA微阵列技术来对单核苷酸多态性（SNP）进行基因型分析。尽管基于3-D聚丙烯酰胺凝胶的微阵列平台是一种简单，可靠且高通量的方法，可在一个样品上检测大量样品的一个SNP对于大型样品，有时需要对更多的SNP进行基因分型。在这项研究中，我们开发了多重聚合酶链反应（multiple-PCR），并结合了基于凝胶的微阵列，该微阵列可以同时对大量样品进行基因分型。通过该方法一次检测到五个GABRA4基因的SNP（rs191296，rs2280073，rs17599165，rs17599416和rs7660336）。我们的实验表明，多重PCR与3-D聚丙烯酰胺凝胶基微阵列相结合可以作为一种简单，可靠，高通量的方法，尤其是节省大量人的许多SNP分析的省时和省钱的方法。

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