• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Forum on Tartary Buckwheat Industrial Economy; 20060909-11; Taiyuan(CN) >DETERMINATION OF RUTIN AND QUERCETIN IN TARTARY BUCKWHEAT BY REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
【24h】

DETERMINATION OF RUTIN AND QUERCETIN IN TARTARY BUCKWHEAT BY REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

机译：反相高效液相色谱法测定苦荞中芦丁和槲皮素

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Object: To establish a Re-HPLC method for the quantitative determination of rutin and quercetin in the flavonoid from Tartary buckwheat. Methods: Detected the concents of hydrolysis and un-hydrolysis sample with rutin and quercetin as the mark sample respectively, used Kromasil C18-column with 0.4% of phosphoric acid-methanol (volume ratio of 40:60) as the mobile phase and detect ed at 358nm, the flow rate was 1.0ml/min, the columntemperature was set at 20°C. Results: The calibration curves were linear in the ranges of 0.025 - 0.125g/L (r = 0.9993) and 0.008- 0.256g/L (r = 0.9995) for rutin and quercetin respectively. The average recoveries were 98.5% and 99.9%; the RSD were 1.16% (n = 5) and 1.50% (n= 5) for rutin and quercetin respectively. In the hydrolysis sample, the concents of quercetin were 194.7 ± 4.22mg/g, RSD was2.17%.In the un-hydrolysis sample, quercetin were 8.96 ± 0.26mg/g, RSD was 2.90% (n = 3), rutin were 83.84 ± 3.29mg/g, RSD Was 3.92% (n = 3 ). Conclusion: This methods are simple, rapid, accurate and reliable. It is useful for determination rutin and quercetin in the flavonoid from Tartary buckwheat.
机译：目的：建立一种HPLC法测定苦荞中黄酮类成分中芦丁和槲皮素的含量。方法：分别以芦丁和槲皮素为标记样品，检测水解和未水解样品的浓度，以0.4％磷酸-甲醇（体积比为40:60）的Kromasil C18柱作为流动相，并进行检测。在358nm下，流速为1.0ml / min，柱温设定为20℃。结果：芦丁和槲皮素的校准曲线分别在0.025-0.125g / L（r = 0.9993）和0.008- 0.256g / L（r = 0.9995）范围内呈线性。平均回收率分别为98.5％和99.9％；芦丁和槲皮素的RSD分别为1.16％（n = 5）和1.50％（n = 5）。水解样品中槲皮素的含量为194.7±4.22mg / g，RSD为2.17％;未水解样品中的槲皮素为8.96±0.26mg / g，RSD为2.90％（n = 3），芦丁为83.84±3.29mg / g，RSD为3.92％（n = 3）。结论：该方法简便，快速，准确，可靠。用于测定苦荞中黄酮类化合物中的芦丁和槲皮素。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号