首页> 外文会议>International Kenaf Symposium on International Development of Kenaf and Allied Fibers; 20030819-21; Beijing(CN) >Establishment of Agrobacterium-mediated Genetic Transformation System of Kenaf and Transfer Target Genes into Kenaf
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Establishment of Agrobacterium-mediated Genetic Transformation System of Kenaf and Transfer Target Genes into Kenaf

机译:农杆菌介导的红麻遗传转化体系的建立及靶基因的导入

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摘要

After optimized factors affecting transformation, an Agrobacterium-mediated genetic transfer method has been established. Some factors influencing transformation efficiency were tested. Based on this system, the target genes of chitinase gene and B.t gene were attempted to transfer into kenaf. The assay of GUS gene expression showed that Chitinase gene and B.t gene were transferred into kenaf line 7804.
机译:在优化影响转化的因素之后,已经建立了农杆菌介导的遗传转移方法。测试了一些影响转化效率的因素。基于该系统,试图将几丁质酶基因和B.t基因的靶基因转移到洋麻中。 GUS基因表达的测定表明几丁质酶基因和B.t基因被转移到洋麻品系7804中。

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