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首页> 外文会议>International Plant Nutrition Colloquium; 2005; Beijing >Cloning and functional analysis of genes involved in root gravitropism in soybean as related to P availability
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Cloning and functional analysis of genes involved in root gravitropism in soybean as related to P availability

机译：与磷有效性相关的大豆根向性相关基因的克隆与功能分析

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Root gravitropism is an important factor determining root architecture, which essentially affects phosphorus (P) uptake and utilization efficiency by soybean plants. In the present study, we attempted to test the hypothesis in which low P availability would lead to the shallowness of whole root system in soybean at molecular biology level. BX10 was used as material representing P deficiency sensitive genotype, grown in specially designed paper pouch culture and treated with low P (0.2 μM), high P (1 mM), stratified P (upper 10 cm layer, 1 mM; 10-30 cm, 0.2 μM ). Genes involved in root gravitropism were homologously cloned in BX 10 roots, and gene expression pattern were analyzed by carrying out Northern blot. Northern results indicated that P availability regulated the expression of some genes. It is interesting to note that all genes regulated by P availability are involved in auxin transport. Our preliminary results implicated that P availability regulated auxin transportation. Since auxin asymmetric distribution is the key step in the whole root gravitropism process, we speculated that P availability may regulate root gravitropism. However, further functional analysis of these genes would be required to reveal the essential mechanisms implied in changes of root gravitropism regulated by P availability.
机译：根向重力作用是决定根结构的重要因素，根结构从根本上影响大豆植物对磷（P）的吸收和利用效率。在本研究中，我们试图检验以下假设：在分子生物学水平上，低磷有效性将导致大豆整个根系变浅。 BX10用作代表对P缺乏敏感基因型的材料，在专门设计的纸袋培养物中生长，并用低P（0.2μM），高P（1 mM），分层P（上层10 cm，1 mM; 10-30 cm）处理，0.2μM）。将与根系引力有关的基因同源克隆到BX 10根中，并通过Northern印迹法分析基因表达模式。 Northern结果表明，磷的有效利用调节了某些基因的表达。有趣的是，所有受磷利用率调节的基因都参与了植物生长素的运输。我们的初步结果表明，磷的可用性调节了植物生长素的运输。由于生长素的不对称分布是整个根向重力过程的关键步骤，我们推测磷的有效性可能调节根向重力。但是，将需要对这些基因进行进一步的功能分析，以揭示由磷有效性调节的根向性改变所隐含的基本机制。

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