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首页> 外文会议>International Society of Sugar Cane Technologists Congress vol.2; 20050130-0204; Guatemala City(GT) >AGROBACTERIUM-MEDIATED TRANSFORMATION OF SUGARCANE (SACCHARUM OFFICINARUM L.) WITH BACTERIAL PHYTASE GENE
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AGROBACTERIUM-MEDIATED TRANSFORMATION OF SUGARCANE (SACCHARUM OFFICINARUM L.) WITH BACTERIAL PHYTASE GENE

机译：农杆菌介导的蔗糖酶基因转化甘蔗（SACCHARUM OFFICINARUM L.）

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摘要

Modern sugarcane cultivars are interspecific hybrids with complex genetic characteristics and low fertility, which render their genetic improvement through traditional breeding difficult. Introducing foreign genes by genetic engineering provides new opportunities for the genetic improvement of sugarcane cultivars. To improve the nutritional quality of sugarcane as animal feed, a bacterial phytase-encoding gene was introduced into sugarcane. Sugarcane was successfully transformed with a new protocol for Agrobacterium-mediated transformation. 72-100% of the calli produced new calli, and 100% of them were positive for the inserted bacterial gene. The whole procedure permitted the production of transgenic callus in short time (1.5 months). Some of the transgenic callus was finally regenerated into plantlets. This is the first report on transformation of sugarcane with a bacterial phytase-encoding gene.
机译：现代甘蔗品种是具有复杂遗传特性和低繁殖力的种间杂种，这使其通过传统育种难以进行遗传改良。通过基因工程引入外源基因为甘蔗品种的遗传改良提供了新的机会。为了提高甘蔗作为动物饲料的营养质量，将一种细菌植酸酶编码基因引入了甘蔗中。用农杆菌介导的转化新方案成功转化了甘蔗。 72-100％的愈伤组织产生了新的愈伤组织，其中100％对插入的细菌基因呈阳性。整个过程允许在短时间内（1.5个月）产生转基因愈伤组织。一些转基因愈伤组织最终再生为小植株。这是关于用细菌植酸酶编码基因转化甘蔗的第一份报告。

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