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首页> 外文会议>International Symposium on Chemical and Biological Sensors and Analytical Methods II, 2001 >WIRING EFFICIENCY IN LAYER-BY-LAYER (PAA-Os)_n(GOx)_n SELF-ASSEMBLED GLUCOSE BIOSENSORS
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WIRING EFFICIENCY IN LAYER-BY-LAYER (PAA-Os)_n(GOx)_n SELF-ASSEMBLED GLUCOSE BIOSENSORS

机译：逐层（PAA-Os）_n（GOx）_n自组装的葡萄糖生物传感器的布线效率

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摘要

Supramolecular structures comprised of glucose oxidase and a poly-cation based on Os complex derivatised poly(allylamine) (PAA-Os) have been constructed by alternate electrostatic adsorption in a layer-by-layer self-assembly process. The resulting modified electrodes were tested as reagentless glucose biosensors. The enzyme kinetic parameters and the surface concentration of "wired" enzyme (Γ_(enz)(wired)) have been obtained by analysis of the catalytic current dependence on glucose concentrations: k[Os], assuming k_(cat) and K_M values for the ping-pong mechanism of glucose oxidase. The osmium volumen concentration has been obtained by integration of the redox charge in the absence of glucose and the ellipsometric thickness of these multilayers. The total enzyme surface concentration was measured with a quartz crystal microbalance in each adsoption step. The amperometric response of the reagentless biosensors has been analysed in terms of the fraction of "wired" enzyme and the bimolecular rate constant for FADH_2 oxidation by the redox polymer for the different multylayers.
机译：由葡萄糖氧化酶和基于Os复杂衍生化聚烯丙胺（PAA-Os）的聚阳离子组成的超分子结构已经通过逐层自组装过程中的交替静电吸附而构建。测试所得的修饰电极作为无试剂葡萄糖生物传感器。酶动力学参数和“有线”酶（Γ_（enz）（wired））的表面浓度已通过分析催化电流对葡萄糖浓度的依赖性获得：k [Os]，假设k_（cat）和K_M值分别为葡萄糖氧化酶的乒乓机制通过在不存在葡萄糖的情况下氧化还原电荷的积分以及这些多层的椭偏厚度获得了volume体积浓度。在每个吸附步骤中，用石英晶体微量天平测量总酶表面浓度。已针对“有线”酶的分数和氧化还原聚合物对不同multylayer的FADH_2氧化的双分子速率常数，分析了无试剂生物传感器的安培响应。

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