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首页> 外文会议>International Symposium on Chemical and Biological Sensors and Analytical Methods II, 2001 >ONLINE MEASUREMENT OF GLUCOSE IN A ROTATING WALL PERFUSED VESSEL BIOREACTOR USING AN AMPEROMETRIC GLUCOSE SENSOR
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ONLINE MEASUREMENT OF GLUCOSE IN A ROTATING WALL PERFUSED VESSEL BIOREACTOR USING AN AMPEROMETRIC GLUCOSE SENSOR

机译：使用安培葡萄糖传感器在线测量旋转壁灌注式血管生物反应器中的葡萄糖

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摘要

A reliable, long-term stable glucose sensor was developed and evaluated for continuous measurement of glucose in a rotating wall perfused vessel (RWPV) bioreactor, where baby hamster kidney cells (BHK-21) were grown. The sensor was based on a hydrogen peroxide electrode system. The sensor was employed to continuously measure the glucose concentration of cell culture media in a RWPV bioreactor for 30 days. The steady-state response time was less than 15 minutes. The output of the sensor was converted to the glucose concentration utilizing the two-point calibration method. The development sensor was able to measure glucose continuously and accurately for 16 days using a single calibration. The glucose measurement error using this sensor was +-15 mg/dl, as measured using the Beckman Glucose Analyzer, in the period of 16 days. The factors, which influence the sensor properties such as pH of the culture media, flow rate and temperature, were also examined.
机译：开发了可靠的长期稳定的葡萄糖传感器，并对其进行了评估，以便在旋转壁灌注容器（RWPV）生物反应器中连续测量葡萄糖，在该反应器中生长了仓鼠肾细胞（BHK-21）。该传感器基于过氧化氢电极系统。该传感器用于连续测量RWPV生物反应器中细胞培养基中葡萄糖的浓度，持续30天。稳态响应时间少于15分钟。利用两点校准方法将传感器的输出转换为葡萄糖浓度。显影传感器能够使用一次校准连续16天准确地测量葡萄糖。使用贝克曼葡萄糖分析仪测得的该传感器在16天内的葡萄糖测量误差为+ -15 mg / dl。还检查了影响传感器特性的因素，例如培养基的pH，流速和温度。

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