首页> 外文会议>International Symposium on Chemical and Biological Sensors and Analytical Methods II, 2001 >SIMULATION OF TRANSIENT ISOTACHOPHORESIS (STACKING) FOR CONCENTRATION OF SAMPLES IN A MICROFLUIDIC DEVICE
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SIMULATION OF TRANSIENT ISOTACHOPHORESIS (STACKING) FOR CONCENTRATION OF SAMPLES IN A MICROFLUIDIC DEVICE

机译:微流体装置中样品的瞬时同位素电泳(痕量)模拟

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Capillary electrophoresis (CE) is one of the methods used in analytical chemistry for separation and detection of charged species due to differences in mobility in an electric field. One of the newer technologies is to implement electrophoresis on a single-use plastic device (so called disposable lab-on-a-chip). The quality of the CE separation can be improved by preconcentrating the sample, which improves the analytical sensitivity and resolution. One method of preconcentration is to use a method called stacking (1-8). In this technique, differences in the conductivity and mobility of the injected sample relative to the background electrolyte (BGE) cause a lower electric field in the injection zone, which subsequently leads to a sharpening of the concentration profile (stacking). We have investigated both sharpening from the trailing edge (back-stacking) and leading edge (front-stacking) of fluorescein in a double-T injector and compared the results to computer simulations.
机译:毛细管电泳(CE)是分析化学中用于分离和检测带电物质的方法之一,这归因于电场中迁移率的差异。一种较新的技术是在一次性塑料设备(所谓的一次性芯片实验室)上实施电泳。通过对样品进行预浓缩可以提高CE分离的质量,从而提高分析灵敏度和分离度。一种预浓缩方法是使用一种称为堆积(1-8)的方法。在此技术中,相对于背景电解质(BGE)而言,进样样品的电导率和迁移率的差异会导致进样区中的电场降低,从而导致浓度分布锐化(堆积)。我们已经研究了在双T型注射器中荧光素的后缘(后堆叠)和前缘(前堆叠)锐化的情况,并将结果与​​计算机模拟进行了比较。

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