The Transfection Efficacy of Chitosan-DNA Nanoparticles on MGC803, SMMC-7721, MG63 cells

机译：壳聚糖-DNA纳米颗粒对MGC803，SMMC-7721，MG63细胞的转染效果

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Chitosan-DNA nanoparticles were synthesized from the complexation of chitosan solution and the plasmid pCD-GFP encoding green fluorescent protein through a coacervation process. The nanoparticles formed were of diameter inferior to 100 nm. The transfection efficacy of chitosan-DNA nanoparticles in MGC803 cells was higher than in SMMC-7721 cells, whereas the plasmid GFP was not expressed in MG63 cells.

机译：壳聚糖溶液和编码绿色荧光蛋白的质粒pCD-GFP通过凝聚作用复合，合成了壳聚糖-DNA纳米颗粒。形成的纳米颗粒的直径小于100nm。壳聚糖-DNA纳米粒在MGC803细胞中的转染效率高于SMMC-7721细胞，而质粒GFP在MG63细胞中不表达。

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来源
《International Symposium on Eco-Materials Processing amp; Design; 20060108-11; Chengdu(CN)》|2006年|P.382-385|共4页
会议地点 Chengdu(CN)
作者
Song Chen; Bin Zhang; Lei Ren; Li-ping Sun; Qi-qing Zhang;
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作者单位

Research Center of Biomedical Engineering, Medical College, Xiamen University, Xiamen 361005, P.R.China;

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会议组织
原文格式 PDF
正文语种 eng
中图分类工程材料一般性问题;
关键词
chitosan; GFP; nanoparticles; transfection;

机译：壳聚糖; GFP;纳米颗粒;转染;

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专利

1. Mesenchymal stem cells, MG63 and HEK293 transfection using chitosan-DNA nanoparticles [J] . Corsi K., Chellat F., Yahia L., Biomaterials . 2003,第7期

机译：使用壳聚糖-DNA纳米颗粒的间充质干细胞，MG63和HEK293转染
2. Chitosan-DNA nanoparticles as non-viral vectors in gene therapy: strategies to improve transfection efficacy. [J] . Mansouri S, Lavigne P, Corsi K, European journal of pharmaceutics and biopharmaceutics: official journal of Arbeitsgemeinschaft fuer Pharmazeutische Verfahrenstechnik e.V . 2004,第1期

机译：壳聚糖-DNA纳米颗粒作为基因治疗中的非病毒载体：提高转染效率的策略。
3. Chitosan-DNA nanoparticles: the effect of cell type and hydrolysis of chitosan on in vitro DNA transfection. [J] . Turan K, Nagata K Pharmaceutical development and technology . 2006,第4期

机译：壳聚糖-DNA纳米颗粒：细胞类型和壳聚糖水解对体外DNA转染的影响。
4. The Transfection Efficacy of Chitosan-DNA Nanoparticles on MGC803, SMMC-7721, MG63 cells [C] . Song Chen, Bin Zhang, Lei Ren, International Symposium on Eco-Materials Processing Design . 2006

机译：壳聚糖-DNA纳米粒子对MGC803，SMMC-7721，MG63细胞的转染效果
5. Chitosan-DNA nanoparticles: Synthesis, characterization, subcellular transport and oral delivery of genetic vaccines. [D] . Roy, Krishnendu. 2000

机译：壳聚糖-DNA纳米颗粒：遗传疫苗的合成，表征，亚细胞运输和口服递送。
6. Subcellular trafficking and transfection efficacy of polyethylenimine-polyethylene glycol polyplex nanoparticles with a ligand to melanocortin receptor-1 [O] . Mikhail O Durymanov, Elena A Beletkaia, Alexey V Ulasov, -1

机译：聚乙烯 - 聚乙二醇多重聚合物纳米粒子用配体与黑色素受体-1的亚细胞运输和转染功效
7. Chitosan-pDNA nanoparticle characteristics determine the transfection efficacy of gene delivery to human mesenchymal stem cells [O] . Malakooty Elham, Esmailnejad .., Baghaban Mohamadreza, 2014

机译：壳聚糖-pDNA纳米颗粒的特性决定了基因传递至人间充质干细胞的转染效率

The Transfection Efficacy of Chitosan-DNA Nanoparticles on MGC803, SMMC-7721, MG63 cells

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