• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Symposium on Medical and Pharmaceutical Biotechnology(医药生物技术国际研讨会) >The construction of eukaryotic expression vector of human decorin and study on its anti-tumor effect in vitro
【24h】

The construction of eukaryotic expression vector of human decorin and study on its anti-tumor effect in vitro

机译：人decorin真核表达载体的构建及其体外抗肿瘤作用的研究

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To construct the eukaryotic expression vector of human decorin and investigate the anti-tumor effect of decorin on sarcoma cells in vitro. The eukaryotic expression vector of recombination pcDNA3. 1 ( + )-DCN was constructed. Recombinant plasmid pcDNA3.1( + )-DCN and empty plasmid pcDNA3. 1 ( + ) were stably transfected into S180 cells by liposome transfection, respectively. G418 of different concentrations was used to screen the positive transformants with RT-PCR being used to determine the expression of DCN gene. The effect of DCN on the cell cycles and apoptosis of S180 cells was analyzed by the flow cytometer. Apoptosis indexes of the group of S180 cells transfected with DCN significantly increased, with the proportion of cells in G0/G1 phase being higher than that of other control groups and the proportion of cells in G2/M and S phase being lower than that of other control groups. Human DCN can induce sarcoma cells apoptosis, which is most occurred in G0/G1 of the cell cycle.
机译：构建人decorin的真核表达载体，研究decorin对肉瘤细胞的体外抗肿瘤作用。重组pcDNA3的真核表达载体。构建了1（+）-DCN。重组质粒pcDNA3.1（+）-DCN和空质粒pcDNA3。通过脂质体转染分别将1（+）稳定转染到S180细胞中。使用不同浓度的G418来筛选阳性转化体，通过RT-PCR确定DCN基因的表达。用流式细胞仪分析了DCN对S180细胞周期和凋亡的影响。转染DCN的S180细胞组的凋亡指数显着增加，G0 / G1期的细胞比例高于其他对照组，G2 / M和S期的细胞比例低于其他对照组。对照组。人DCN可以诱导肉瘤细胞凋亡，其最常见于细胞周期的G0 / G1。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号