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首页> 外文会议>International Water Association(IWA) Conference; 20050518-20; Xi'an(CN) >Microbial community dynamics during start-up of bio-hydrogen producing reactor
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Microbial community dynamics during start-up of bio-hydrogen producing reactor

机译：生物制氢反应堆启动过程中的微生物群落动态

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A laboratory-scale continuous hydrogen production is carried on by using molasses as its feed. During these 35 days of the start-up period, microbial community dynamics are monitored by using the denaturing gradient gel electrophoresis (DGGE) analysis of the V3 region of 16S rRNA genes. After running 7-14days, there is an obvious change in the dominant populations of the microbial community. And this change accompanies with a change of fermentation type. The dominant populations are as follows low G+C gram-positive bacteria, Bacteroides, Actinobacteria and Y-Proteobacteria, respectively. Most of the hydrogen producing bacteria are Clostridium sp., Bacteroides sp., Acetanaerobacterium sp., Megasphaera sp., Bacteroides sp., and Enterobacter sp., respectively. The community structure composition and the amount of dominant population are different when the reactor operates in different periods. Different niche conditions lead to different enrichment of bacteria, and forms different microbial community structures.
机译：通过使用糖蜜作为进料进行实验室规模的连续制氢。在启动的这35天中，通过使用16S rRNA基因V3区的变性梯度凝胶电泳（DGGE）分析来监测微生物群落动态。运行7-14天后，微生物群落的优势种群发生了明显的变化。并且这种变化伴随着发酵类型的变化。优势种群分别为低G + C革兰氏阳性细菌，拟杆菌，放线菌和Y-变形杆菌。大部分产氢细菌分别是梭菌属，拟杆菌属，醋杆菌属，巨噬菌属，拟杆菌属和肠杆菌属。反应堆在不同时期运行时，群落结构组成和优势种群数量不同。不同的生态位条件导致不同的细菌富集，并形成不同的微生物群落结构。

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