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首页> 外文会议>Journal of Anhui Agricultural University >Characterization of the Orchid Mycorrhizal Fungi Isolated from roots of Korean Native Orchids
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Characterization of the Orchid Mycorrhizal Fungi Isolated from roots of Korean Native Orchids

机译：从韩国本土兰花的根中分离出的兰花菌根真菌的特性

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The orchid symbioticfungi were isolated an identified from the roots of korean native orchidsusing modern molecular techniques. The selected isolates were segregated into several groups based on microscopic observtions and PCR-RAPD results, and symbiosis responses were different for the different fungi o nthe various plant species. All fungi isolates selctedstimulated the root growth for all orchid species tested, and some affected the shoot growth fo the cymbidium temperate irchid. the five fungi 18s rDNA sequences were analyzed using BLAST. Based on the tested different characteristics, the fungal isolates were segregated into three groups. Two of these three symbiotic isolate groups were confirmed as Rhizoctonia repens(P01) and rhizoctonia endophytica var. endophytica(P02). The patterns of the dendrograms made based on sequences of 18s rDNA were consistent with patterns of dendrograms obtained from the plant responses, but not ocnsistent with the polymorphisms indicated by PCR-RAPD. The fungal isolates collected from C.goeringii, a Korean native orchid, were different from plant pathogenic species fo Rhizoctonia an Fusarium, wel known as a soil borne disease. Also, the techniques to distinguish the symbiotic isolate from the pathogenic fungus was developed by suing the PCR-RAPD reacted with the primers employed.
机译：使用现代分子技术从韩国原生兰花的根中分离出一种兰花共生真菌。根据显微镜观察和PCR-RAPD结果，将选定的分离株分为几类，不同植物的不同真菌的共生反应也不同。所有真菌分离物都刺激了所有兰花品种的根生长，有些影响了温带大花ym兰的枝条生长。使用BLAST分析了五个真菌18s rDNA序列。根据测试的不同特征，将真菌分离物分为三类。这三个共生分离株中有两个被确认为白质致病菌（R01）和内生真菌。内生菌（P02）。基于18s rDNA序列构建的树状图谱与从植物反应获得的树状图谱一致，但与PCR-RAPD指示的多态性不一致。从韩国本土兰花C.goeringii收集的真菌分离物不同于镰刀菌根瘤菌的植物病原体，被称为土壤传播疾病。同样，通过使用与所用引物反应的PCR-RAPD，开发了将共生分离物与病原性真菌区分开的技术。

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