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首页> 外文会议>Journal of Huazhong Agricultural University （ Ziran Kexue Ban ) >Phylogenetic Relationships of Pleurotus species Based on rDNA PCR-RFLP
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Phylogenetic Relationships of Pleurotus species Based on rDNA PCR-RFLP

机译：基于rDNA PCR-RFLP的白灵菇菌种亲缘关系

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摘要

28S rDNA 5′ half, ITS and IGS1 region of eighteen Pleurotus taxa, together with Agaricus bis-porus, Lentinula edodes and Hohenbuehelia serotina were amplified using PCR and then digested with seven restriction endonucleases. A single uniform 1.46 kb product resulted from PCR amplification of 28S rDNA 5′ half of four genera. Amplification of ITS resulted in a single 680 bp product for Pleurotus, 700 bp for H. serotina, 720 bp for L. edodes and A. bisporus. The length of IGS1 was varied: a 1.1 kb product for P. djamor and P. salmoneostramineus, a common product 1.0 kb for the other Pleurotus isolates, 700 bp for H. serotina, 1.3 kb for L. edodes and A. bisporus. Dendrogram based on PCR-RFLP of 28S rDNA 5′ half, ITS and IGS1 suggested that H. serotina, L. edodes and A. bisporus could distinguish from Pleurotus, the isolates of Pleurotus were divided into five groups; P. tube-rregium, P. djamor and P. salmoneostramineus, P. abalonus and P. cystiodisus, P. citri-nopileatus, the other isolates clustered together. P. abalonus and P. cystidiosus were the same species, as well as P. djamor and P. salmoneostramineus, P. pulmonarius and P. sajor-caju. rDNA PCR-RFLP was more suitable for taxonomy and phylogenetic relationships of Pleurotus.
机译：利用PCR扩增了18个平菇类群的28S rDNA 5'一半，ITS和IGS1区域，以及双孢蘑菇，香菇和Hohenbuehelia serotina，然后用7种限制性核酸内切酶消化。 PCR扩增四个属的一半的28S rDNA 5'，得到一个单一的1.46 kb均匀产物。 ITS的扩增导致白灵菇单一的680 bp产物，血清嗜血杆菌700 bp，香菇和双孢蘑菇720 bp。 IGS1的长度各不相同：假单胞菌和鲑鱼链霉菌的产物为1.1 kb，其他平菇分离株的产物为1.0 kb，血清志贺菌为700 bp，香菇和双孢曲霉为1.3 kb。根据28S rDNA 5'一半的PCR-RFLP，ITS和IGS1进行的树状图表明，H。serotina，L。edodes和A. bisporus可以与白灵菇区分开，将白灵菇菌株分为五组。 P. tube-rregium，P。djamor和P.鲑鱼曲霉，P。abalonus和P. cystiodisus，P。citri-nopileatus，其他分离株聚集在一起。 Abalonus和P. cystidiosus是同一物种，以及djamor和P. saleoeostramineus，P。pulmonarius和P. sajor-caju。 rDNA PCR-RFLP更适合平菇的分类学和系统发育关系。

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