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Orthogonal Experiment on the Optimum Culture Conditions of Paecilomyces cicadae

机译:蝉拟青霉最佳培养条件的正交试验

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The optimum culture conditions of Paecilomyces cicadae were investigated by the orthogonal experiment in three factors and three levels of nutrient concentration. The basic culture conditions were the 5% of inoculation amount, 120 r/min, at 28℃ and for 120 h. The optimum culture condition was selected by testing and analyzing dry weight of mycelium, which was C_6 H_(12) O_6 (glucose) 20 g/ L, yeast extract 5 g/ L, K_2 HPO_4 1 g/L. The carbon source was the most important nutrient factor for the growth of Paecilomyces cicadae.
机译:通过正交试验,在三个因素和三个水平的养分浓度水平下,研究了蝉拟青霉的最佳培养条件。基本的培养条件是在28℃下120 h / min接种量的5%(120 r / min)。通过测试和分析菌丝体干重来选择最佳培养条件,其为C_6 H_(12)O_6(葡萄糖)20 g / L,酵母提取物5 g / L,K_2 HPO_4 1 g / L。碳源是蝉拟青霉生长最重要的营养因子。

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