Laser-Induced Fluorescence Imaging System for Protein Separations in Microfluidic Devices

机译：激光诱导荧光成像系统，用于微流控设备中的蛋白质分离

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This paper describes a laser-induced fluorescence (LIF) detection system for imaging proteins separated in a microfluidic device. The diameter of a laser beam is first increased through a beam expander, and subsequently focused into a line using a cylindrical lens. The resultant laser line is used to image an entire capillary or channel in which protein separation took place. The fluorescence emission is collected with a cooled, scientific grade charge-coupled device (CCD) camera. The detection limit was determined using a series of concentrations of fluorescein solutions. The temporal and spatial effects of photobleaching from laser irradiation were analyzed and the parameters to reduce the effect of photobleaching are discussed. We used the imaging system to demonstrate rapid analysis of proteins using isoelectric focusing.

机译：本文介绍了一种激光诱导荧光（LIF）检测系统，用于对微流控设备中分离的蛋白质进行成像。首先通过扩束器增加激光束的直径，然后使用圆柱透镜将其聚焦成一条线。产生的激光线用于对发生蛋白质分离的整个毛细管或通道成像。用冷却的科学级电荷耦合器件（CCD）摄像机收集荧光发射。使用一系列浓度的荧光素溶液确定检测限。分析了激光辐照光漂白的时间和空间效应，并讨论了降低光漂白效应的参数。我们使用成像系统演示了使用等电聚焦对蛋白质进行的快速分析。

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来源
《Lab-on-a-Chip: Platforms, Devices, and Applications》|2004年|P.190-197|共8页
会议地点 PhiladelphiaPA (US)
作者
Champak Das; Alexer Stoyanov; Carl Fredrickson; Roger Tran-Son-Tay; Z. Hugh Fan;
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作者单位

Department of Mechanical Aerospace Engineering University of Florida, P.O. Box 116250, Gainesville, FL 32611;

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会议组织
原文格式 PDF
正文语种 eng
中图分类半导体技术;
关键词
laser-induced fluorescence (LIF); isoelectric focusing (EEF); microfluidics; whole-channel imaging; green fluorescent protein (GFP);

机译：激光诱导荧光（LIF）;等电聚焦（EEF）;微流控;全通道成像;绿色荧光蛋白（GFP）;

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专利

1. A low-cost, low-power consumption, miniature laser-induced fluorescence system for DNA detection on a microfluidic device. [J] . Shrinivasan S, Norris PM, Landers JP, Clinics in laboratory medicine . 2007,第1期

机译：一种低成本，低功耗的微型激光诱导荧光系统，用于在微流体设备上进行DNA检测。
2. A Low-Cost, Low-Power, Consumption Miniature Laser-Induced Fluorescence System for DNA Detection on a Microfluidic Device [J] . Sushil Shrinivasan, Pamela M. Norris, James P. Landers, Journal of Laboratory Automation . 2006,第4期

机译：低成本，低功耗，微型激光诱导荧光系统，用于微流控设备上的DNA检测
3. Measurement of pH field of chemically reacting flow in microfluidic devices by laser-induced fluorescence [J] . Kyosuke Shinohara, Yasuhiko Sugii, Koji Okamoto, Measurement Science & Technology . 2004,第5期

机译：激光诱导荧光测量微流控装置中化学反应流的pH场
4. Laser-induced fluorescence imaging system for protein separations in microfluidic devices [C] . Champak Das, Alexander Stoyanov, Carl Fredrickson, Conference on Lab-on-a-Chip . 2004

机译：用于微流体装置中的蛋白质分离的激光诱导的荧光成像系统
5. Genome analysis: Mutation analysis using near-infrared laser-induced fluorescence (NIR-LIF) and single molecule detection in microfluidic devices. [D] . Wabuyele, Musundi Ben. 2003

机译：基因组分析：使用近红外激光诱导的荧光（NIR-LIF）和微流控设备中的单分子检测进行突变分析。
6. Microfluidics on the fly: Inexpensive rapid fabrication of thermally laminated microfluidic devices for live imaging and multimodal perturbations of multicellular systems [O] . Megan Levis, Nilay Kumar, Emily Apakian, 2019

机译：飞行中的微流控技术：便宜的快速制造热层压微流控设备用于实时成像和多细胞系统的多峰扰动
7. Development of Microfluidic Devices with the Use of Nanotechnology to Aid in the Analysis of Biological Systems Including Membrane Protein Separation, Single Cell Analysis, and Genetic Markers [O] . Goldberg Mark David 2015

机译：利用纳米技术开发微流体装置，帮助分析包括膜蛋白分离，单细胞分析和遗传标记在内的生物系统
8. Rapid Protein Separations in Microfluidic Devices [R] . Fan, Z. H. , Das, C. , Xia, Z. , 2004

机译：微流体装置中的快速蛋白质分离

Laser-Induced Fluorescence Imaging System for Protein Separations in Microfluidic Devices

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