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SHG microscopy excited by polarization controlled beam for three-dimensional molecular orientation measurement

机译：偏振控制光束激发的SHG显微镜用于三维分子取向测量

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We have developed a second-harmonic-generation (SHG) microscope to observe the three-dimensional molecular orientation with three-dimensional high spatial resolution using a polarization mode converter. The mode converter consists of a parallel-aligned nematic-liquid-crystal spatial-light-modulator (PAL-SLM) and quarter-waveplates, and converts a incident linearly polarized beam to orthogonal linearly polarized beams or radially polarized beam. We combined the mode converter with SHG microscope to obtain the local information of the three-dimensional molecular orientation. We demonstrated the detection of three-dimensional molecular orientation of collagen fiber in human Achilles' tendon. For high precision three-dimensional molecular orientation measurement, we propose a technique to calibrate the dependence of SHG detection efficiencies on molecular orientation using a liposome.

机译：我们已经开发了第二谐波产生（SHG）显微镜，以使用偏振模式转换器以三维高空间分辨率观察三维分子取向。模式转换器由平行排列的向列液晶空间光调制器（PAL-SLM）和四分之一波片组成，并将入射的线性偏振光束转换为正交线性偏振光束或径向偏振光束。我们将模式转换器与SHG显微镜相结合，以获得三维分子取向的局部信息。我们证明了人类跟腱的胶原纤维三维分子取向的检测。对于高精度三维分子取向测量，我们提出了一种使用脂质体来校准SHG检测效率对分子取向的依赖性的技术。

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来源
《Laser Beam Shaping VII》|2006年|P.62900F.1-62900F.8|共8页
会议地点 San DiegoCA(US)
作者
K. Yoshiki; M. Hashimoto; T. Araki;
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作者单位

Organization for the Promotion of Research on Nanoscience Nanotechnology, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, JAPAN;

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会议组织
原文格式 PDF
正文语种 eng
中图分类激光技术、微波激射技术;
关键词
second harmonic generation; molecular orientation measurement; spatial polarization control; spatial light modulator;

机译：二次谐波;分子取向测量;空间偏振控制;空间光调制器;

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专利

1. Second-Harmonic-Generation Microscopy Using Excitation Beam with Controlled Polarization Pattern to Determine Three-Dimensional Molecular Orientation [J] . Keisuke YOSHIKI, Mamoru HASHIMOTO, Tsutomu ARAKI Japanese Journal of Applied Physics. Part 2, Letters & Express Letters . 2005,第33a36期

机译：二次谐波产生显微镜，使用控制偏振模式的激发光束确定三维分子取向
2. Three-dimensional orientation measurements of symmetric single chromophores using polarization microscopy [J] . S. A. Empedocles, R. Neuhauser, M. G. Bawendi Nature . 1999,第6732期

机译：使用偏振显微镜对对称单发色团的三维取向测量
3. Proposition of single molecular orientation determination using polarization controlled beam by liquid crystal spatial light modulators [J] . Hashimoto M, Yamada K, Araki T Optical review . 2005,第1期

机译：液晶空间光调制器利用偏振控制光束确定单分子取向的建议
4. SHG microscopy excited by polarization controlled beam for three-dimensional molecular orientation measurement [C] . K. Yoshiki, M. Hashimoto, T. Araki, Conference on Laser Beam Shaping . 2006

机译：SHG显微镜通过极化控制光束激发三维分子取向测量
5. Implementation of three-dimensional SHG imaging microscopy for tissue characterization and disease diagnostics: Experiment and simulations. [D] . LaComb, Ronald B. 2010

机译：用于组织表征和疾病诊断的三维SHG成像显微镜的实现：实验和模拟。
6. Room temperature measurements of the 3D orientation of single CdSe quantum dots using polarization microscopy [O] . Inhee Chung, Ken T. Shimizu, Moungi G. Bawendi 2003

机译：使用偏振显微镜在室温下测量单个CdSe量子点的3D方向
7. 1P174 Three-dimensional orientation measurements of single molecules by newly developed polarized fluorescence microscopy(Molecular motor,The 48th Annual Meeting of the Biophysical Society of Japan) [O] . Masashi Ohmachi, Yasunori Komori, Toshio Yanagida 2010

机译：1P174通过新开发的偏振荧光显微镜（分子电机，日本生物物理学会第48次年度会议的单分子的三维取向测量
8. Raman, Transmission Electron Microscopy, and Conductivity Measurements in Molecular Beam Deposited Microcrystalline Si and Ge. A Comparative Study [R] . Gonzalez-Hernandez, J., Azarbayejani, G. H., Tsu, R., 1985

机译：拉曼，透射电子显微镜和分子束沉积微晶硅和锗的电导率测量。比较研究

SHG microscopy excited by polarization controlled beam for three-dimensional molecular orientation measurement

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