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Automated laser spectrofluorimeter for monitoring of myocardial metabolism

机译:自动激光荧光分光光度计监测心肌代谢

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摘要

Methods of optical biopsy have a series of advantages before other methods of clinical diagnostics. The high accuracy of received results enables registration even small change of concentration of substances, and the opportunity of remote registration makes methods optical biopsy by an optimum means for noninvasive methods of diagnostics in medicine. The method of the fluorescent analysis allows to investigate dynamics of changes of a functional condition of organs and tissue in norm and pathologies, called by the various factors (an inflammation, ischemia, degenerative changes). Bring the results of development of expiremental setup for the laser fluorescent analysis of physiological and functional condition of various organs and tissue of organism. In expiremental setup was used pulse UF nitric laser with length of wave generation = 337 nm. For delivery of radiation to tissue, and, also, collection of a radiation of fluorescence were used various optic fiber scheme. The expiremental setup includes automated tunable monochromator and ADC, receiving a signal from photomultiplier tube. Driving of all blocks and processing of results is realize on IBM-compatible computer with the appropriate software. Was used the synchronous detecting for reducing of a background signal. Myocard at surgical introoperation by an accompanied condition of sharp ischemia was researches on these expiremental setup. Spectrofluorimetric criteria of an estimation of a condition of viscus at peritonitis were development.
机译:在其他临床诊断方法之前,光学活检方法具有一系列优势。接收结果的高精度使得即使物质浓度的微小变化也可以进行配准,并且远程配准的机会使光学活检方法成为医学诊断中非侵入性方法的最佳手段。荧光分析的方法允许研究器官和组织的功能状况在规范和病理学中的动态变化,这些变化被各种因素(炎症,局部缺血,退化性变化)称为。带来过期设置发展的结果,对生物体各器官和组织的生理和功能状况进行激光荧光分析。在过期设置中,使用脉冲超滤硝酸激光,其产生波的长度= 337 nm。为了将辐射递送到组织,并且还使用了各种光纤方案来收集荧光辐射。过期设置包括自动可调单色仪和ADC,从光电倍增管接收信号。使用适当的软件在IBM兼容计算机上实现所有块的驱动和结果处理。用于减少背景信号的同步检测。在外科手术中伴随着严重缺血的心肌是对这些过期设置的研究。建立了估计腹膜炎内脏状况的荧光光谱标准。

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