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Optical trapping forces calculation by dielectrophoresis

机译：介电泳法计算光俘获力

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In this work we present a laser trapping system based on an Ar~+ laser and the calibration method we used for the optical trapping force measurements on polystyrene spheres. The method uses the dielectrophoretic force exerted on a dielectric particle from an inhomogeneous electric field for the calculation of the optical force. We used polystyrene microspheres on a dielectrophoretic chamber with planar electrodes in the optical tweezers setup. For the determination of the dielectrophoretic force the method is based on Green's theorem. A sphere is trapped in the optical tweezers at various laser powers. An electrical field is applied between the electrodes and thus a dielectrophoretic force is exerted on the particle. The electric field is increased very slowly until the cell is pulled out from the trap. At this moment the optical force equals the dielectrophoretic force, which can be theoretically calculated. This algorithm is repeated several times and the optical force is graphically represented as a function of laser power. Forces of some pN were calculated.
机译：在这项工作中，我们提出了一种基于Ar〜+激光器的激光捕获系统，以及我们用于测量聚苯乙烯球上的光学捕获力的校准方法。该方法使用来自不均匀电场施加在介电粒子上的介电泳力来计算光学力。我们在光镊装置中在带有平面电极的介电泳室中使用了聚苯乙烯微球。为了确定介电泳力，该方法基于格林定理。用各种激光功率将球体困在光镊中。在电极之间施加电场，从而在颗粒上施加介电泳力。电场的增加非常缓慢，直到将细胞从阱中拉出为止。此时，光学力等于介电泳力，这可以从理论上计算出来。将该算法重复多次，并将光学力图形表示为激光功率的函数。计算出一些pN的力。

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