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Laser-induced autofluorescence spectroscopy of benign and dysplastic nevi and malignant melanoma

机译:激光诱导的自发荧光光谱的良性和异常增生性痣和恶性黑色素瘤

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摘要

The easy and non-destructive fluorescence method for quantification of early changes in biological tissues improves the possibilities of the clinical research. Using endogenous and exogenous fluorophores one can achieve high accuracy in the determination of malignant melanoma lesions with wide clinical applications. The goals of this work were investigation of melanin-pigmented cutaneous lesions by the methods of laser-induced autofluorescence spectroscopy (LIAFS), using nitrogen laser (337 nm) as excitation source. All lesions investigated were excised and investigated histologically by standard methods and histological results were used for comparison with the spectra detected. In the cases of nevi and melanoma significant decrease of fluorescence intensity toward normal skin fluorescence, which correlated with the type of pigment lesion was observed. The results, obtained in this investigation of the different pigment lesions could be used for better comprehension of the skin optical properties. The fluorescence spectroscopy of the human skin is very prominent for early diagnosis and differentiation of cutaneous diseases.
机译:用于量化生物组织早期变化的简便且无损的荧光方法提高了临床研究的可能性。使用内源性和外源性荧光团可以在确定恶性黑色素瘤病变方面获得高精度,具有广泛的临床应用。这项工作的目标是通过使用氮气(337 nm)作为激发源,通过激光诱导自发荧光光谱(LIAFS)的方法研究黑色素染色的皮肤病变。通过标准方法切除所有研究的病灶并进行组织学研究,并使用组织学结果与检测到的光谱进行比较。在痣和黑色素瘤的情况下,观察到朝向正常皮肤荧光的荧光强度显着降低,这与色素病变的类型相关。在这项研究中获得的不同色素损伤的结果可用于更好地理解皮肤的光学特性。人类皮肤的荧光光谱对于皮肤疾病的早期诊断和鉴别非常重要。

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