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首页> 外文会议>Management of Classical Swine Fever and Foot-and-Mouth Disease in Lao PDR >Application of immunomagnetic bead technology for improved diagnosis of classical swine fever virus
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Application of immunomagnetic bead technology for improved diagnosis of classical swine fever virus

机译：免疫磁珠技术在经典猪瘟病毒诊断中的应用

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Classical swine fever (CSF) is a highly contagious viral disease of swine that causes major losses in all pig production systems in many regions of the world. In Lao PDR, CSF is endemic and outbreaks have adverse effects on the predominantly smallholder farming sector. Laboratory testing is required to accurately identify CSF outbreaks because of the difficulty of making a diagnosis based solely on clinical signs. The National Animal Health Centre, located in the national capital, Vientiane, has the capacity to reliably detect CSF antigen in tissue samples using an antigen capture (AC)-ELISA, and antibodies to CSF virus from serum samples using the complex trapping blocking ELISA. This paper describes the use of immunomagnetic beads (1MB) as the solid phase for the portable detection of CSF antigen in spleen samples and for the reliable detection of antibodies to CSF in animals vaccinated with a lapinised C-strain vaccine. The portable IMB-ELISA for antigen detection was shown to be 100% sensitive and 91% specific in comparison to the AC-ELISA. The IMB-Antibody-ELISA was shown to be 97% sensitive and 95% specific in comparison to the gold standard-neutralising peroxidase linked assay. These new diagnostic tests have the potential to improve CSF management through portable and rapid identification of outbreaks and the reliable and inexpensive monitoring of vaccination programs.
机译：古典猪瘟（CSF）是一种高度传染性的猪病毒性疾病，在世界许多地区的所有生猪生产系统中造成重大损失。在老挝人民民主共和国，脑脊液是地方病，疫情对以小农为主的农业部门产生不利影响。由于难以仅根据临床体征做出诊断，因此需要进行实验室测试以准确识别脑脊液暴发。位于国家首都万象的国家动物卫生中心具有使用抗原捕获（AC）-ELISA可靠检测组织样品中CSF抗原的能力，并使用复杂的捕获阻断ELISA方法可从血清样品中可靠地检测CSF病毒抗体。本文描述了免疫磁珠（1MB）作为固相用于便携式检测脾脏样品中的CSF抗原以及可靠地检测接种了Lapinized C株疫苗的动物中CSF抗体的用途。与AC-ELISA相比，用于抗原检测的便携式IMB-ELISA具有100％的敏感性和91％的特异性。与金标准中和的过氧化物酶连锁检测法相比，IMB-抗体-ELISA法显示出97％的敏感性和95％的特异性。这些新的诊断测试具有潜力，可以通过便携式，快速地识别疫情以及对疫苗接种计划进行可靠且廉价的监控来改善CSF管理。

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