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Polarized Light Improves Cutaneous Healing on Diabetic Rats

机译：偏振光可改善糖尿病大鼠的皮肤愈合

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The aim of this study was to evaluate the healing of 3~rd degree burn on diabetic rats submitted or not to treatment with Polarized Light Diabetes mellitus (Streptozotocin, 60mg/kg) was induced on 45 male Wistar albinus rats and a third degree burn (1.5X l.Scm) was created in the dorsum of each animal under general anesthesia. After a regular quarantine period, the animals were randomly distributed into three groups as follows: Gl: control (no treatment, n =15); G2: Polarized Light (λ=400-2000nm, 20J/cm~2) and G3: Polarized Light (X,=400-2000nm, 40J/cm~2). The phototherapy performed on group G2 was Polarized Light dose 20J/cm2 and G3 was Polarized Light dose 40J/cm2 (Bioptron~?, λ400-2000 nm, 40mW; 2.4J/cm~2 per minute; Φ ± 5.5 cm; Bioptron AG, Monchaltorf, Switzerland). The phototherapy started immediately post-burning and was repeated daily until the day before the animal death. The energy was applied transcutaneously respecting the focal distance of 10cm as recommended by the manufacturer. The dose was 20 or 40J/cm~2 (4min 15s or 8min.and 30s). At each time point chosen (7, 14, and 21 days post-burning) and following macroscopic examination, each animal was killed by an overdose of general anesthesia. Slides were stained with HE, Sirius Red, and CK AE1/AE3 antibody. Qualitative and semi-quantitative analyses were performed under light microscopy. The animals submitted to phototherapy (20J/cm~2) showed significant differences on regards revascularization and epithelialization. The use of 20J/cm~2 was effective on improving the healing of third degree buns on diabetic animals at both early and late stages of the repair.
机译：本研究的目的是评估对45只雄性Wistar白化病大鼠诱发或未接受偏光性糖尿病（链脲佐菌素，60mg / kg）治疗的糖尿病大鼠三度烧伤的愈合情况。在全身麻醉下，在每只动物的背部产生1.5×1.Scm。在常规的检疫期后，将动物随机分为三组，如下所述：G1：对照（未处理，n ＝ 15）；和。 G2：偏振光（λ＝ 400-2000nm，20J / cm〜2），G3：偏振光（X，＝ 400-2000nm，40J / cm〜2）。 G2组的光疗为偏光剂量20J / cm2，G3偏光剂量为40J / cm2（Bioptron〜λ，λ400-2000nm，40mW; 2.4J / cm〜2每分钟;Φ±5.5 cm; Bioptron AG ，瑞士蒙恰托夫）。燃烧后立即开始光疗，每天重复一次，直到动物死亡。按照制造商的建议，以10cm的焦距经皮施加能量。剂量为20或40J / cm〜2（4min 15s或8min.30s）。在选定的每个时间点（燃烧后7、14和21天）并进行宏观检查后，每只动物均被过量的全身麻醉杀死。将玻片用HE，Sirius Red和CK AE1 / AE3抗体染色。在光学显微镜下进行定性和半定量分析。接受光疗（20J / cm〜2）的动物在血运重建和上皮形成方面表现出显着差异。在修复的早期和晚期，使用20J / cm〜2均可有效改善糖尿病动物的三级面包的愈合。

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