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Direct measurement of nitric oxide concentration in isolated vessels and isolated beating hearts by nitric oxide microsensor

机译:一氧化氮微传感器直接测量离体血管和离体跳动心脏中一氧化氮的浓度

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There is much value to be obtained from evaluating the real-time change of nitric oxide (NO) in cardiovascular systems, since NO plays roles in various physiological and pathophysiological phenomena, including vasorelaxation and atherosclerosis. The authors thus applied a new NO microsensor (100 /spl mu/m/spl phi/), which allows one to directly measure NO release on a real-time basis with high temporal and spatial resolutions, to the measurement of NO in isolated arteries and hearts where NO plays key roles. The NO concentration (measured current) in canine femoral arterial wall increased linearly with increasing perfusion flow rate. It was also increased by acetylcholine and decreased by N/sup G/-nitro-L-arginine. The NO concentration in the coronary effluent of isolated beating rat hearts also increased with increasing perfusion flow rate and showed transient changes during ischemia-reperfusion.
机译:通过评估心血管系统中一氧化氮(NO)的实时变化,可以获得很多价值,因为NO在包括血管舒张和动脉粥样硬化在内的各种生理和病理生理现象中发挥着作用。因此,作者应用了一种新型的NO微传感器(100 / spl mu / m / spl phi /),该传感器可以实时测量具有高时空分辨率的实时NO释放,用于测量孤立动脉中的NO。和没有NO发挥关键作用的心。犬股动脉壁中的NO浓度(测量电流)随灌注流量的增加而线性增加。它也被乙酰胆碱增加而被N / sup G /-硝基-L-精氨酸减少。离体搏动的大鼠心脏冠状流出物中的NO浓度也随灌注流量的增加而增加,并在缺血-再灌注过程中表现出短暂的变化。

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