• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议> >Inactivation of biofilm-forming bacteria using cold atmospheric plasmas and potential application for decontamination of fresh foods
【24h】

Inactivation of biofilm-forming bacteria using cold atmospheric plasmas and potential application for decontamination of fresh foods

机译：使用冷大气等离子体灭活形成生物膜的细菌及其在新鲜食品净化中的潜在应用

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Summary form only given. This work aims at evaluating the prospects for using cold atmospheric plasmas to decontaminate fresh produce. Nonthermal atmospheric plasmas have mainly been studied in the past as methods for killing pathogens on surfaces either in medical or environmental contexts. Most of these studies are based on cell inactivation on surfaces such as glass, plastics and metals which do not usually support cell growth. In food decontamination, there is the complication of interaction occurring between microbial cells and the surface. This interaction can take many different forms: micro-organisms may adsorb to the surfaces of food and simply remain attached to it during processing as is the case with abiotic materials. Alternatively, adherent organisms may exploit nutrients present on the surface of foods and go on to proliferate at the surface. Others may, under certain conditions, exude polysaccharides which serve to protect them from environmental stresses and, incidentally, makes it more difficult to detach them from surfaces. This three-dimensional matrix of polysaccharides and micro-organisms is referred to as a 'biofilm.' In order to facilitate rigorous evaluation of our decontamination methods, we have chosen to conduct our initial experiments using a model system. In future work once we have optimised the operation of our plasma, we intend to use naturally contaminated foods and additionally, to evaluate the effects of cold plasmas on key nutrients in the food. Our model system comprises the biofilm-forming bacterium Pantoea (Enterobacter) agglomerans growing on synthetic membranes to simulate bacterial growth on the surface of plant tissue. As the biofilm develops with time, the production of polysaccharides becomes more extensive and cells contained within the matrix are shielded to an increasing degree. We present data on the kinetics of inactivation of Pantoea agglomerans in biofilms of different ages by cold atmospheric plasmas generated in a helium-oxygen mixture and with a radio-frequency excitation. Data is also presented on the UV treatment of such biofilms in order to permit comparison of both methods of food decontamination.
机译：仅提供摘要表格。这项工作旨在评估使用冷大气等离子体净化新鲜农产品的前景。过去，非热大气等离子体主要作为在医学或环境方面杀死表面病原体的方法进行了研究。这些研究大多数基于玻璃，塑料和金属等通常不支持细胞生长的表面上的细胞失活。在食品净化中，微生物细胞与表面之间会发生相互作用的复杂情况。这种相互作用可以采取许多不同的形式：微生物可能会吸附到食物的表面，并在加工过程中像非生物材料那样简单地保持附着在食物上。另外，粘附的生物可能会利用食物表面存在的营养物，然后继续在食物表面扩散。在某些条件下，其他化合物可能会散发出多糖，这些多糖可保护它们免受环境压力的影响，并因此使它们从表面分离变得更加困难。多糖和微生物的三维矩阵称为“生物膜”。为了便于对我们的去污方法进行严格的评估，我们选择使用模型系统进行初始实验。在我们优化血浆操作之后的未来工作中，我们打算使用天然污染的食品，此外，还要评估冷血浆对食品中关键营养素的影响。我们的模型系统包含在合成膜上生长的生物膜形成细菌泛菌（肠杆菌），以模拟细菌在植物组织表面的生长。随着生物膜的发展，多糖的产生变得更加广泛，基质中所含的细胞被越来越多地屏蔽。我们提出了在不同年龄的生物膜中，通过氦-氧混合物中产生的冷大气等离子体和射频激发而灭活泛菌的动力学数据。还提供了有关对此类生物膜进行紫外线处理的数据，以便比较两种食品去污方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号