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Hepatitis C Virus Genotyping and Serum Anti-F Antibody Detection of HCV Patients in the South of Jiangsu Province of China

机译：苏南地区丙型肝炎病毒丙型肝炎病毒基因分型和血清抗F抗体检测

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摘要

This study investigated the distribution of hepatitis C virus (HCV) genotypes and assessed the prevalence and distribution of serum anti-F in patients with hepatitis C virus (HCV) infection in the south of Jiangsu province of China. One hundred and fifty-eight anti-HCV positive sera samples were indentified by the PCR with type specific primers designed according to the sequence of 5'-non-coding region (5' NCR). Indirect ELISA was carried to detect anti-F in sera of 120 HCV patients and 10 donors. Ninty-five HCV RNA positive patients (95/158) were classified as 80 (80/95, 84.2%) genotype 1b, 5 (5/95, 5.3%) genotype 2, 5 (5/95, 5.3%) genotype 1b/2 mixed and 5 (5/95, 5.3%) genotype undetermined. Then genotype 1b was the predominant in the south of jiangsu province of China. The difference on the distribution of HCV genotypes was significant between female and male patients(p<0.05), but not in different kinds of HCV patients. Eighty-two samples (68%) out of the 120 HCV infected patients exhibited a positive anti-F reaction, showing significant difference from the controls without HCV infection (p<0.01). Chi-square test showed that there were no significant correlations between anti-F antibody and sex, HCV RNA serum, types of HCV RNA. Patients of midrange, severe range and hepatic cirrhosis have higher rate than others(p<0.05), the positive rate of anti-F is higher in patients over fifties(p<0.01). This report of the serum anti-F prevalence and distribution of anti-F in the South of Jiangsu Province hepatitis C patients may help the investigation of the pathogenesis of HCV, and functions of F protein.
机译：本研究调查了丙型肝炎病毒（HCV）基因型的分布，并评估了中国南部南部乙型肝炎病毒（HCV）感染患者血清抗F的患病率和分布。通过根据5'-非编码区（5'NCC）的序列设计的特异性引物，通过PCR识别一百五十八个抗HCV阳性血清样品。携带间接ELISA以检测120个HCV患者和10个供体的血清中的抗F.第五个HCV RNA阳性患者（95/158）分类为80（80/95,84.2％）基因型1b，5（5/95,5.3％）基因型2,5（5/95,5.3％）基因型1b / 2混合和5（5/95,5.3％）基因型未确定。然后基因型1B是中国江苏省南部的主要素。 HCV基因型分布的差异是雌雄和男性患者（P <0.05）之间的显着性，但不含不同种类的HCV患者。 120个HCV感染患者中的八十二个样品（68％）表现出阳性抗F反应，显示出与没有HCV感染的对照有显着差异（P <0.01）。 Chi-Square测试表明，抗F抗体和性别，HCV RNA血清，HCV RNA类型之间没有显着相关性。患者的中间患者，严重的范围和肝硬化的速率高于其他速率（P <0.05），抗F的阳性率在五十年代患者较高（P <0.01）。江苏省丙型肝炎患者南部血清抗F患病率和抗F的分布的报告可能有助于调查HCV的发病机制，以及F蛋白的功能。

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