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Microfluidic device for rapid detection of cytomegalovirus (CMV) by sequence-specific hybridization of PCR-amplified CMV-DNA

机译：通过PCR扩增的CMV-DNA的序列特异性杂交快速检测巨细胞病毒（CMV）的微流体装置

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This paper reports rapid detection of human CMV by using a microfluidic device fabricated on plastic chip. The method employs post PCR product analysis by sequence-specific hybridization between amplified CMV-DNA target and complementary PNA probe in microchannel for specific detection of CMV. The PCR product solution and PNA probe solution flowed simultaneously along the microchannel forming a laminar flow at the straight channel. Hybridization of PCR amplified target DNA and fluorescently labeled peptide nucleic acid (PNA) probe occurred at the interface of the laminar flow. Secondary laminar flow, on the other hand, is formed at the curving part of the microchannel allowing separation of DNA hybrids. Hybridized DNA were detected by laser induced fluorescence microscopy. Collectively, these features allowed identification of PCR amplified CMV-DNA. Compared to the conventional pp65 antigenemia test, microfluidic device is found to be more sensitive in detecting low-level viremia
机译：本文报道了通过使用在塑料芯片上制造的微流控设备快速检测人类CMV的方法。该方法通过在微通道中扩增的CMV-DNA靶标和互补PNA探针之间进行序列特异性杂交来进行PCR后产物分析，从而对CMV进行特异性检测。 PCR产物溶液和PNA探针溶液同时沿微通道流动，在直通道处形成层流。 PCR扩增的目标DNA和荧光标记的肽核酸（PNA）探针的杂交发生在层流的界面处。另一方面，次级层流在微通道的弯曲部分形成，从而可以分离DNA杂种。通过激光诱导荧光显微镜检测杂交的DNA。总而言之，这些特征允许鉴定PCR扩增的CMV-DNA。与传统的pp65抗原血症测试相比，发现微流体装置对检测低水平病毒血症更为敏感

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