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An AFM Study of the Hierarchical DNA Immobilization/Hybridization Processes on Surfaces

机译：表面上分层DNA固定/杂交过程的AFM研究

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摘要

The immobilization and hybridization processes of DNA strands on poly-1-lysine (PL) covered surfaces have been studied using the atomic force microscopy (AFM) in a topographic mode. The statistical analysis of topographic surfaces showed an increase in the Z-threshold with additions of single strand DNA (ssDNA) and the complimentary DNA (ccDNA). Also no drastic change of statistical fractal dimension (slope of the log-log perimeter-area plot) is observed when comparing the PL-surfaces coated with ssDNA and ccDNA. These two results suggest that ssDNA strands are successfully immobilized and spatially hybridized with ccDNA on the PL surface and the growth of hybridized ccDNA occurs mainly in the vertical dimension. The methods described here are good candidates for the detection of DNA hybridization, especially in the context of DNA nanoarrays.
机译：DNA链在聚1-赖氨酸（PL）覆盖的表面上的固定和杂交过程已使用原子力显微镜（AFM）以地形图模式进行了研究。地形表面的统计分析表明，Z阈值随单链DNA（ssDNA）和互补DNA（ccDNA）的增加而增加。当比较涂有ssDNA和ccDNA的PL表面时，也没有观察到统计分形维数（对数对数周长-面积图的斜率）的急剧变化。这两个结果表明，ssDNA链已成功固定在PL表面并与ccDNA在空间上杂交，并且杂交ccDNA的生长主要发生在垂直方向。这里描述的方法是检测DNA杂交的良好候选者，尤其是在DNA纳米阵列的情况下。

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