Preparation of lipid modified lipase for interesterification and hydrolysis of oils

机译：用于油脂酯交换和水解的脂质修饰脂肪酶的制备

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Lipase modification by addition of lipid is a simple and effective way to greatly improve enzyme activity for n-hexane based interesterification and hydrolysis reactions. A range of modifying lipids and lipases were evaluated, with stearic acid and lipase Saiken 100 (Rhizopus japonicus) investigated in more detail. Enzyme protein recovery and activity were influenced by the quantity of stearic acid addition and pH of the aqueous preparation phase. Modified lipase protein was characterized used SDS-PAGE electrophoresis.

机译：通过添加脂质来修饰脂肪酶是一种简单有效的方法，可以大大提高基于正己烷的酯交换反应和水解反应的酶活性。用硬脂酸和脂肪酶Saiken 100（Rhizopus japonicus）进行了更详细的研究，评估了一系列的修饰脂质和脂肪酶。硬脂酸的添加量和水性制剂相的pH值影响酶蛋白的回收率和活性。用SDS-PAGE电泳鉴定修饰的脂肪酶蛋白。

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《Asia-Pacific biochemical engineering conference;APBIOCHEC'97》|1997年|p.485-488|共4页
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Sosaku Ichikawa; Kenneth D Green; Mitsutoshi Nakajima; Ken-ichi Mogi;
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中图分类生物化学;
关键词
modified lipase; interesterification; hydrolysis; stearic acid; oils and fats;

机译：修饰的脂肪酶酯化水解;硬脂酸;油脂;

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1. Preparation of acidic lipase immobilized surface-modified mesoporous activated carbon catalyst and thereof for the hydrolysis of lipids [J] . K. Ramani, R. Boopathy, A.B. Mandal Catalysis Communications . 2011,第1期

机译：固定化酸性脂肪酶的表面改性介孔活性炭催化剂及其脂质的水解
2. Synthesis of Structured Lipids by Lipase-Catalyzed Interesterification of Triacetin with Camellia Oil Methyl Esters and Preliminary Evaluation of their Plasma Lipid-Lowering Effect in Mice [J] . Yu Cao, Suijian Qi, Yang Zhang Molecules . 2013,第4期

机译：山茶油甲基酯经脂肪酶催化三醋精酯交换合成结构化脂质及其对小鼠降血脂作用的初步评价
3. Synthesis of Structured Lipids by Lipase-Catalyzed Interesterification of Triacetin with Camellia Oil Methyl Esters and Preliminary Evaluation of their Plasma Lipid-Lowering Effect in Mice [J] . Bo Yang, Suijian Qi, Xiaoning Wang, Molecules . 2013,第4期

机译：山茶油甲基酯经脂肪酶催化三醋精酯交换合成结构化脂质及其对小鼠降血脂作用的初步评价
4. Preparation of lipid modified lipase for interesterification and hydrolysis of oils [C] . Sosaku Ichikawa, Kenneth D Green, Mitsutoshi Nakajima, Asia-Pacific biochemical engineering conference . 1997

机译：脂质改性脂肪酶的制备以获取脂质改性和水解油
5. Catalysis of the interesterification reactions of octanoic acid and the triglycerides of olive oil and milkfat by an immobilized lipase [D] . Reyes, Hector R. 1991

机译：固定化脂肪酶催化辛酸与橄榄油和乳脂中甘油三酸酯的酯交换反应
6. Lipase catalyzed interesterification of Amazonian patauá oil and palm stearin for preparation of specific-structured oils [O] . Paula Speranza, Ana Paula Badan Ribeiro, Gabriela Alves Macedo 2015

机译：脂肪酶催化亚马孙patauá油和棕榈硬脂精的酯交换反应用于制备特定结构的油
7. Evaluation of Lipid Modified Lipase for Interesterification and Hydrolysis Reactions in n-Hexane. [O] . Kenneth D. GREEN, Mitsutoshi NAKAJIMA, Sosaku ICHIKAWA, 1997

机译：N-己烷中脂质改性脂肪酶的评价及水解反应。

Preparation of lipid modified lipase for interesterification and hydrolysis of oils

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