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首页> 外文会议>Asia-Pacific biochemical engineering conference;APBIOCHEC'97 >Production of poly-beta-hydroxybutyrate and measurement of relevant enzyme activities in methylosinus trichosporium OB3b
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Production of poly-beta-hydroxybutyrate and measurement of relevant enzyme activities in methylosinus trichosporium OB3b

机译：聚三羟甲基毛孢子菌OB3b中聚β-羟基丁酸酯的生产和相关酶活性的测定

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摘要

Production of poly-beta-hydroxybutyric acid (PHB) and three key enzymes related to its synthesis, beta-ketothiolase (beta-KT), NADPH-dependent acetoacetyl-CoA reductase (AAR), and citrate synthase (CS) were studied in an obligatory methanotroph Methylosinus trichosporium OB3b. When cultivated in a two-stage mode, the initial growth-stage in a complete medium and the following production-stage in a nitrate-deficient medium, M. trichosporium OB3b showed a significant difference in the cellular PHB level depending on the medium Cu availability during its growth. When Cu was available, the level was more than 50 percent of the dry mass and this was accompanied by a low CS activity and a high beta-KT activity. When Cu was deficient, on the other hand, PHB accumulation was only 12 percent and enzyme activities were the opposite.
机译：研究了聚-β-羟基丁酸（PHB）及其合成相关的三种关键酶β-酮硫解酶（β-KT），NADPH依赖性乙酰乙酰辅酶A还原酶（AAR）和柠檬酸合酶（CS）的生产。必食甲烷营养菌甲基毛孢子虫OB3b。当以两阶段模式进行培养时，在完全培养基中的初始生长阶段和在硝酸盐缺乏培养基中的后续生产阶段，毛孢霉OB3b在细胞PHB水平上表现出显着差异，具体取决于培养基中Cu的可用性在其成长过程中。当可获得铜时，其含量超过干重的50％，同时伴有较低的CS活性和较高的β-KT活性。另一方面，当铜缺乏时，PHB的积累仅为12％，而酶的活性却相反。

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