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首页> 外文会议>Biotechnology for Fuels and Chemicals >Production and Characterization of Phanerochaete chrysosporium Lignin Peroxidases for Pulp Bleaching
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Production and Characterization of Phanerochaete chrysosporium Lignin Peroxidases for Pulp Bleaching

机译：纸浆漂白Phanerochaete chsssporium木质素过氧化物酶的生产与表征

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The production of lignin peroxidase from Phanerochaete chrysosporium was studied using immobilized mycelia in nylon-web cubes in semiconti-nuous fermentation using glucose pulses or ammonium tartrate pulses. Consistent enzyme production was achieved when glucose pulses were used, leading to an average activity of 253 U/L. The crude enzyme was added to eucalyptus kraft pulp before conventional and ECF bleaching sequences. Optimization of the enzymatic pretreatment led to the following operational conditions: enzyme load of 2 U/g of pulp, hydrogen peroxide addition rate of 10 ppm/h, and reaction time of 60 min. Pulp final characteristics were dependent on the chemical treatment sequence that followed enzymatic pretreatment. The chief advantage of enzymatic pretreatment was pulp viscosity preservation, which was observed in most of the experiments carried out with seven different chemical treatment sequences.
机译：使用葡萄糖脉冲或酒石酸铵脉冲在半连续发酵中使用固定化菌丝体在尼龙网立方体中研究了从Phanerochaete chrysosporium产生木质素过氧化物酶的方法。当使用葡萄糖脉冲时，可实现一致的酶产生，从而导致平均活性为253 U / L。在常规和ECF漂白程序之前，将粗酶添加到桉木牛皮纸浆中。酶促预处理的优化导致以下操作条件：2 U / g纸浆的酶负载，10 ppm / h的过氧化氢添加速率和60分钟的反应时间。纸浆的最终特性取决于酶促预处理后的化学处理顺序。酶法预处理的主要优势是保持纸浆粘度，这在使用七个不同化学处理顺序进行的大多数实验中都可以观察到。

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