Presence of Aeromonas hydrophila in waters in Indiana: a field and pilot study

机译：印第安那州水中嗜水气单胞菌的存在：一项实地研究和初步研究

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Enumeration of Aeromonas hydrophila was performed using Ampicillin-Dextrin Agar. Biochemical characterization of environmental isolates included the use of API and Biolog detection methods, whereas blood agar hemolysis was used to test for virulence. Enumeration was conducted in source water, in various sites within a water treatment plant, and in the distribution system. The presence of A. hydrophila was studied in both bulk water and biofilm within full scale and simulated (annular reactors) drinking water distribution systems.The study was conducted over a one-year period. Presumptive Aeromonas sp. counts on source waters approached 10~3 CFU/mL during summer months and filtered water counts ranged from <1 to 168 CFU/100mL. Also, 4.3% of the plant effluent samples (after disinfection), 11.7% of the distribution bulk water samples and 21.0 % of the biofilm samples provided presumptive Aeromonas sp results. Furthermore, A. hydrophila was definitively identified in 10.5% of the biofilm samples, but never in the treatment plant effluent or distributed bulk water, showing disinfectant efficiency on suspended bacteria. On the other hand, the presence of A. hydrophila in biofilms within the distribution system and positive isolates from simulated systems indicate potential for regrowth and contamination in drinking water distribution systems.

机译：使用氨苄青霉素-糊精琼脂进行嗜水气单胞菌的计数。环境分离物的生化特性包括使用API和Biolog检测方法，而血琼脂溶血则用于测试毒力。枚举是在原水，水处理厂内的各个场所以及分配系统中进行的。在大规模和模拟（环形反应器）饮用水分配系统中的散装水和生物膜中都研究了嗜水链球菌的存在。该研究进行了一年的时间。推定的气单胞菌在夏季，源水的总计数接近10〜3 CFU / mL，过滤水的计数范围从<1至168 CFU / 100mL。同样，有4.3％的植物废水样品（消毒后），分布的散装水样品的11.7％和生物膜样品的21.0％提供了推测的气单胞菌菌种结果。此外，在10.5％的生物膜样品中明确鉴定出嗜水链球菌，但从未在污水处理厂的废水中或散布的散装水中发现过，这显示出对悬浮细菌的消毒效率。另一方面，在分配系统内的生物膜中存在嗜水链球菌以及从模拟系统中分离出阳性分离物，这表明饮用水分配系统中有再生和污染的可能性。

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《Water quality technology conference;WQTC》|1999年|p.1-9|共9页
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Christian Chauret; John Jarosh; Graduate Student; Christian Volk; Jeff Robinson;
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Presence of Aeromonas hydrophila in waters in Indiana: a field and pilot study

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