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Biodegradation of 2,4-dichlorophenoxy acetic acid under field conditions in wheat crop

机译：田间条件下小麦作物中2,4-二氯苯氧基乙酸的生物降解

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Experiment was conducted to study the degradation of 2,4-dichlorophenoxy acetic acid (2,4-D) by Azotobacter chroococcum strain 33 and its effects on wheat (cv. WH-283, sensitive to 2,4-D) growth and yield. Wheat seeds wre inoculated with culture of A.chroococcum Strain 33. Crop was sprayed with two concentrations of 2,4-D (ester) viz., d_1 circlea 570 and d_2 circlea of 1140 g a.i. per hectare at 35 days after sowing (DAS).Degradation of 2,4-D and microbial count were followed in the soil collected from the rhizosphere on different days (0,7,14,21,28) after spraying. It was found that 2,4-D degraded at a faster rate in case of inoculation in comparison to that in which inoculation was not carried out. Application ofnitrogen circlea of 90 kg ha~(-1) also enhanced the degradation rate of 2,4-D. Microbial count in soil samples increased with inoculation. Spraying of 2,4-D and application of nitrogen increased soil microbial count. The degradation rate of 2,4-D was found to be at a faster rate under higher dose of it and where inoculation was carried out along with nitrogen application. Seed inoculation with A. chroococcum strain 33, with or without nitrogen applicationincreased growth and yield of wheat. It was also recorded that seed inoculation lessened the adverse effect of both the concentrations of 2,4-D on wheat growth and yield in comparison to non-inoculation.
机译：进行了实验，研究了绿脓杆菌33菌株对2,4-二氯苯氧基乙酸（2,4-D）的降解及其对小麦（cv。WH-283，对2,4-D敏感）的生长和产量的影响。 。小麦种子接种了33.A.chroococcum菌株的培养物。用两种浓度的2,4-D（酯），即d_1circa 570和d_2circa 1140g a.i喷洒了作物。播种后第35天（DAS）的每公顷土地面积。喷施后不同天数（0,7,14,21,28）的根际土壤中的2,4-D降解和微生物数量随之而来。发现与不进行接种的情况相比，在接种的情况下2，4-D以更快的速率降解。施用90 kg ha〜（-1）的氮气圈也提高了2,4-D的降解率。接种后土壤样品中的微生物数量增加。喷洒2,4-D和施氮可增加土壤微生物数量。发现在较高剂量下2,4-D的降解速率更快，并且在接种接种的同时施氮。接种或不施氮的ch.ocrocum菌株33的种子接种增加了小麦的生长和产量。还记录到，与不接种相比，接种种子减轻了2，4-D浓度对小麦生长和产量的不利影响。

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